Analysis of Some Putative Novel Peptides from Iranian Scorpion Venom Glands, <i>Hemiscorpius lepturus</i>, Using cDNA Library Construction
| Author | Amir Jalali | en |
| Author | Masoumeh Baradaran | en |
| Author | Hamid Galehdari | en |
| Author | Parnian Ghoncheh | en |
| Author | Maryam Naderi Soorki | en |
| Orcid | Amir Jalali [0000-0002-4516-2973] | en |
| Orcid | Masoumeh Baradaran [0000-0003-2521-2344] | en |
| Orcid | Hamid Galehdari [0000-0001-6281-4809] | en |
| Orcid | Maryam Naderi Soorki [0000-0003-1477-013X] | en |
| Issued Date | 2023-02-28 | en |
| Abstract | Background: To date, more than 300,000 distinct peptides have been identified in scorpion venom. However, only a limited number of these peptides have been described. Objectives: We characterized some putative peptides from the venom gland cDNA library of the Iranian yellow scorpion Hemiscorpius lepturus. Methods: Total RNA was extracted from yellow Iranian scorpion glands. Single-stranded cDNA (sscDNA) and double-stranded cDNA (dscDNA) were synthesized by polymerase chain reaction (PCR). A cDNA library was achieved by inserting dscDNA into a special vector and subsequently transformed to chemically competent Escherichia coli as a host. The library was screened by culturing the liquid library on Lysogeny broth (LB)-agar plates. Analysis of positive clones was performed by plasmid extraction and the sequencing of the inserts. Finally, all cDNA sequences were analyzed and characterized by bioinformatics software. Results: One hundred colonies were randomly analyzed. Eighty-nine cDNA sequences had acceptable quality for bioinformatics analysis. Five sequences were selected for further analysis. The peptides related to these sequences were divided into two groups, non-disulfide bridge peptides (NDBP) and disulfide bridge peptides (DBP), the application of which in health and medical issues has been suggested. Conclusions: The data obtained in this study may be an important resource for further in vivo and in vitro functional assays to identify valuable therapeutic peptides. | en |
| DOI | https://doi.org/10.5812/jjnpp-133423 | en |
| URI | https://brieflands.com/journals/jjnpp/articles/133423 | en |
| Keyword | cDNA Library | en |
| Keyword | Iranian Scorpion | en |
| Keyword | <i>Hemiscorpius lepturus</i> | en |
| Keyword | Toxin | en |
| Keyword | Pharmaceutical Properties | en |
| Publisher | Brieflands | en |
| Title | Analysis of Some Putative Novel Peptides from Iranian Scorpion Venom Glands, <i>Hemiscorpius lepturus</i>, Using cDNA Library Construction | en |
| Type | Research Article | en |