The Efficacy of Multiplex PCR in Comparison with Agglutination and ELISA in Diagnosis of Human Brucellosis

Abstract

Objective: Brucellosis is a zoonotic disease of which diagnosis is based on clinical symptoms and positive laboratory findings. Since serology tests are not specific and sensitive enough, polymerase chain reaction (PCR) can be an alternative method in making the final decision in suspicious cases. In this study, three diagnostic methods were compared in suspected patients with brucellosis in the endemic area of Mianeh, Iran.