Assessment of Treg Cells CD4(+)CD25(+) in Chronic Cirrhotic Liver Disease and Hepatocellular Carcinoma Egyptian Patients
| Author | Maha A. El Bassuoni | en |
| Author | Mones A. Obada | en |
| Author | Tarek Korah | en |
| Author | Sawsan El Sayed | en |
| Issued Date | 2008-09-30 | en |
| Abstract | Background and Aims: Dysfunction of the host immune system in cancer patients can be due to a number of factors, including secretion of tumor-derived immunosuppressive factors or induction of immune tolerance against tumor-specific antigens. Several studies suggest that suppression of tumor-associated antigen reactivates lymphocytes by CD4+ CD25+ regulatory T (Treg) cells. This study was designed to evaluate whether CD4+CD25+ Treg cells in chronic liver disease and hepatocellular carcinoma (HCC) patients exhibit an expanded Treg pool and to correlate it with liver tumor markers and grading. Methods: Blood samples were collected from 20 patients with cirrhotic liver disease (CLD), 15 HCC patients and 10 healthy control subjects. Alpha feto-protein (AFP), HBV and HCV antibodies were detected by EIA. HCV was confirmed by immunoblotting and RT-PCR. To evaluate HCC grading, abdominal ultrasound guided liver biopsy was done. Patients were categorized into moderately differentiated (grade II) and poorly differentiated (grade III) groups. Cytometric analysis of CD4+CD25+ Treg cells in PBMCs was performed using anti-CD3, anti-CD4, anti-CD25, anti-CD45RA, and IgG-isotype control (FITC and PE). Results: Both CLD and HCC groups were 80% positive for HCV while only 20% of CLD and 11% of HCC patients were positive for HBV. The mean percentage of CD4+CD25+T cell population demonstrated a highly significant increase in comparing HCV to HCC patients [2.47±0.66 vs. 8.96±1.38 (P | en |
| DOI | https://doi.org/ | en |
| URI | https://brieflands.com/journals/hepatmon/articles/70024 | en |
| Keyword | CD4(+)CD25(+) Treg Cell | en |
| Keyword | HCV | en |
| Keyword | Hepatocellular Carcinoma | en |
| Keyword | Liver Cirrhosis | en |
| Publisher | Brieflands | en |
| Title | Assessment of Treg Cells CD4(+)CD25(+) in Chronic Cirrhotic Liver Disease and Hepatocellular Carcinoma Egyptian Patients | en |
| Type | Research Article | en |
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