The Effect of Noscapine on Oxygen-Glucose Deprivation on Primary Murine Cortical Neurons in High Glucose Condition
| Author | Gelareh Vahabzadeh | en |
| Author | Soltan-Ahmed Ebrahimi | en |
| Author | Nahid Rahbar-Roshandel | en |
| Author | Massoud Mahmoudian | en |
| Issued Date | 2016-04-30 | en |
| Abstract | In the present work we set out to investigate the neuroprotective effects of noscapine (0.5-2 µM) in presence of D-glucose on primary murine foetal cortical neurons after oxygen–glucose deprivation/24 h. recovery. Cell viability, nitric oxide production and intracellular calcium ((ca2+)i) levels were evaluated by MTT assay, the modified Griess method and Fura-2 respectively. 25 and 100 mM D-glucose could, in a concentration dependent manner, improve cell viability and decrease NO production and (ca2+)i level in neuronal cells after ischemic insult. Moreover, pre-incubation of cells with noscapine, noticeably enhanced protective effects of 25 and 100 mM D-glucose compared to similar conditions without noscapine pre-treatment. In fact, noscapine attenuated NO production in a dose-dependent fashion, after 30 minutes (min) OGD, during high-glucose (HG) condition in cortical neurons. Pretreatment with 2 μM noscapine and 25 or 100 mM D-glucose, was shown to decrease the rise in (ca2+)i induced by Sodium azide/glucose deprivation (chemical OGD) model. These effects were more pronounced than that of 25 or 100 mM D-glucose alone. | en |
| DOI | https://doi.org/10.22037/ijpr.2016.1844 | en |
| Keyword | Noscapine | en |
| Keyword | D-glucose | en |
| Keyword | intracellular calcium levels | en |
| Keyword | NO | en |
| Keyword | Oxygen-Glucose Deprivation | en |
| Publisher | Brieflands | en |
| Title | The Effect of Noscapine on Oxygen-Glucose Deprivation on Primary Murine Cortical Neurons in High Glucose Condition | en |
| Type | Original Article | en |