Effects of 810 nm and 940 nm Diode Laser on ROS Production in Macrophages: The Role of Interferon-Gamma
| Author | Milad Sadeghi | en |
| Author | Reyhaneh Shoorgashti | en |
| Author | Simin Lesan | en |
| Author | Sadegh Hasannia | en |
| Author | Taraneh Farrokhnia | en |
| Orcid | Reyhaneh Shoorgashti [0000-0002-1658-8983] | en |
| Orcid | Simin Lesan [0000-0003-3877-328X] | en |
| Orcid | Sadegh Hasannia [0000-0002-9964-3182] | en |
| Orcid | Taraneh Farrokhnia [0000-0001-6938-4098] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: Reactive oxygen species (ROS) are critical molecules produced during cellular metabolism. Macrophages, as essential immune cells, play a pivotal role in the immune response by producing inflammatory mediators and releasing ROS. Interferon-gamma (IFN-γ) is a key stimulator of macrophage activity, enhancing ROS production. Objectives: With the increasing application of low-level laser therapy (LLLT) in medicine, this study aims to compare the effects of 810 nm and 940 nm diode laser irradiation on ROS production in macrophages. Methods: This in vitro study utilized a monocyte/macrophage cell line. Cells were seeded into microwell plates and exposed to diode laser irradiation at 810 nm and 940 nm, with power settings of 0.4 watts (energy density 7 - 15 J/cm2) and irradiation times of 15 and 30 seconds. The IFN-γ was added post-irradiation. The ROS production was assessed using a fluorometric method, measuring nitric oxide (NO) as an indirect indicator. Results: The comparison between 810 nm and 940 nm wavelengths showed no significant difference in ROS production (P > 0.05). However, higher energy density (4 J vs. 2 J, P = 0.001), longer irradiation time (30 s vs. 15 s, P = 0.001), and the presence of IFN-γ (P < 0.001) significantly increased ROS levels. Conclusions: This study showed that both 810 nm and 940 nm diode lasers significantly increase ROS production in macrophage cells compared to a control group, despite no significant differences between the two wavelengths. | en |
| DOI | https://doi.org/10.5812/jrps-160276 | en |
| URI | https://brieflands.com/journals/jrps/articles/160276 | en |
| Keyword | Free Radicals | en |
| Keyword | Interferon-gamma | en |
| Keyword | Lasers | en |
| Keyword | Macrophages | en |
| Keyword | Phagocytosis | en |
| Keyword | Reactive Oxygen Species | en |
| Publisher | Brieflands | en |
| Title | Effects of 810 nm and 940 nm Diode Laser on ROS Production in Macrophages: The Role of Interferon-Gamma | en |
| Type | Research Article | en |
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