The Modulating Effect of Carumopticum on Human Peripheral Blood T-Cells Radiation Toxicity

Abstract

Herbs that possess immune modulating properties may be useful in reducing the risk of various radiation induced outcome in radiation therapy patients. The carumopticum extract has now been investigated for its effects on radiation induced apoptosis in blood cells. This study proposes a flowcytometry based method that uses fluorescein-annexin V binding FITC and propidium Iodide to enumerate viable, necrotic, and early and late apoptotic cells within specific subsets of a heterogeneous culture. Blood samples from healthy adult man with no mutagens materials contact history was collected in heparinized tubes. The peripheral blood T-cells that were isolated from blood samples cultured and irradiated while Carumopticum (Zenian in persian) extract was added to aquatic culture media just an hour before radiation treatment. So the irradiated cells underwent apoptosis and apoptotic cells were enumerated by annexin-V protocol and multicolor flow cytometry.The results suggest that Carumopticum(ajowan) extract can potentiate the radiation induced apoptosis of PBLCs(73%).The results suggest that Carumopticum (ajowan) extract can enhance the radiation toxicity in blood T‑cells (P < 0.05).