Cytotoxicity of Fractions of <i>Dracontium spruceanum</i> Chloroform Extract in MDA-MB-231 and MCF-7 Breast Cancer Cell Lines
| Author | Arlem Rodriguez-Huamani | en |
| Author | Maria Medina-Calderon | en |
| Author | Santiago Alvarez-Vega | en |
| Author | Salyoc Tapia-Rojas | en |
| Author | Alejandro Fukusaki-Yoshizawa | en |
| Author | Ana Mayanga-Herrera | en |
| Orcid | Arlem Rodriguez-Huamani [0000-0001-9164-1847] | en |
| Orcid | Maria Medina-Calderon [0000-0002-3617-2563] | en |
| Orcid | Santiago Alvarez-Vega [0000-0001-9579-3258] | en |
| Orcid | Salyoc Tapia-Rojas [0000-0002-7735-3336] | en |
| Orcid | Alejandro Fukusaki-Yoshizawa [0000-0001-5479-8415] | en |
| Orcid | Ana Mayanga-Herrera [0000-0002-4633-9857] | en |
| Issued Date | 2024-08-31 | en |
| Abstract | Background: Breast cancer is a major health issue responsible for numerous deaths worldwide. Medicinal plants are rich in compounds with potential anticancer properties. Objectives: This study aimed to assess the cytotoxicity of fractions from the Dracontium spruceanum chloroform extract on MDA-MB-231 and MCF-7 breast cancer cell lines. Methods: Eleven fractions were screened for cytotoxic effects on cell viability using the MTT assay. Subsequently, the two most cytotoxic fractions were selected, and the half-maximal inhibitory concentration (IC50) was calculated using two-fold dilution concentrations ranging from 100 to 1.56 µg/mL. Results: Fractions F43 and F50 showed the highest cytotoxicity at 100 µg/mL. The IC50 of F43 was 270.8 ± 18.07 µg/mL and 133.0 ± 17.99 µg/mL for MDA-MB-231, and 252.2 ± 21.94 µg/mL and 144.3 ± 23.14 µg/mL for MCF-7 cells at 24 and 48 hours, respectively. For F50, IC50 values were 107.2 ± 2.97 µg/mL and 126.1 ± 14.77 µg/mL for MDA-MB-231, and 236.4 ± 58.7 µg/mL and 104.5 ± 10.54 µg/mL for MCF-7 at corresponding times. Conclusions: Dracontium spruceanum fractions demonstrated moderate cytotoxicity on MDA-MB-231 and MCF-7 breast cancer cell lines, indicating their anticancer potential. Further research is required to identify the metabolites responsible for this activity and to elucidate the molecular mechanisms involved. | en |
| DOI | https://doi.org/10.5812/jjnpp-145843 | en |
| Keyword | Breast Cancer | en |
| Keyword | Cell Viability | en |
| Keyword | Chromatography | en |
| Keyword | Chloroform | en |
| Keyword | Medicinal Plants | en |
| Publisher | Brieflands | en |
| Title | Cytotoxicity of Fractions of <i>Dracontium spruceanum</i> Chloroform Extract in MDA-MB-231 and MCF-7 Breast Cancer Cell Lines | en |
| Type | Research Article | en |