Detection of <i>Brucella</i> spp. in Dairy Products by Real-Time PCR
| Author | Elham Moslemi | en |
| Author | Mohammad Mehdi Soltandalal | en |
| Author | Mohammad Reza Beheshtizadeh | en |
| Author | Afsoon Taghavi | en |
| Author | Hamidreza Kheiri Manjili | en |
| Author | Reza Mahmoudi Lamouki | en |
| Author | Amir Izadi | en |
| Issued Date | 2018-01-31 | en |
| Abstract | Background and Objectives: Brucella is an intracellular gram-negative bacterium that can infect many kinds of mammals like humans, sheep, cattle, etc. Brucellosis is a contagious occupational disease caused by Brucella spp. that affects individuals who have close contact with infected animals. The clinical features of Brucellosis are not disease-specific and almost every organ can be affected. This zoonotic disease is a great health concern and economically important in many countries, such as Iran. The aim of this study was to detect Brucella spp. in pasteurized and non-pasteurized dairy products. Methods: In this study, 208 samples, including goat, sheep, and cow raw and pasteurized milk as well as pasteurized and non-pasteurized cheese, were collected in Tehran province. The DNA was extracted, and then the real-time PCR was used for detection of the Brucella spp. gene. Results: The prevalence of Brucella spp. contamination in the dairy products was: 45.5% in goat`s raw milk, 39.1% in non-pasteurized cheese, 27.3% in sheep`s raw milk, 26.3% in cow`s raw milk, 25% in pasteurized cheese, and 14.7% in pasteurized milk. Conclusions: Rapid and exact detection of pathogens in dairy products is the most significant factor to prevent foodborne diseases. In addition, the real-time PCR assay is sensitive and specific enough to detect a low number of Brucella spp. in dairy products. | en |
| DOI | https://doi.org/10.5812/archcid.12673 | en |
| Keyword | Real-Time PCR | en |
| Keyword | Brucellosis | en |
| Keyword | Pasteurization | en |
| Keyword | Dairy Products | en |
| Keyword | <i>Brucella</i> spp. | en |
| Publisher | Brieflands | en |
| Title | Detection of <i>Brucella</i> spp. in Dairy Products by Real-Time PCR | en |
| Type | Research Article | en |
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