Polydatin Relieves Nociceptive Pain: Possible Role of L-arginine/NO/cGMP/K<sub>ATP</sub> Channel Signaling Pathway in Mice
| Author | Sadaf Abdian | en |
| Author | Sajad Fakhri | en |
| Author | Fatemeh Abbaszadeh | en |
| Author | Mohammad Mehdi Gravandi | en |
| Author | Mohammad Hosein Farzaei | en |
| Author | Amir Kiani | en |
| Orcid | Sajad Fakhri [0000-0001-8265-8284] | en |
| Orcid | Mohammad Mehdi Gravandi [0000-0002-7995-5285] | en |
| Orcid | Mohammad Hosein Farzaei [0000-0001-7081-6521] | en |
| Issued Date | 2025-11-30 | en |
| Abstract | Background: The role of the L-arginine (L-Arg)/nitric oxide (NO)/cyclic GMP (cGMP)/ATP-sensitive potassium channel (KATP) channel pathway in pain regulation is well-known. Previous studies have confirmed the pain-relieving effects of polydatin, but the exact mechanisms behind anti-nociception are not fully understood. Objectives: This study aimed to investigate how the L-Arg/NO/cGMP/KATP channel pathway mediates the pain-relieving effects of polydatin in mice. Methods: The formalin test was employed to assess nociceptive pain. Mice were administered several compounds before receiving intraperitoneal doses of polydatin. The compounds included L-Arg, a precursor of NO; N(gamma)-nitro-l-arginine methyl ester (L-NAME), an inhibitor of NO synthase; and S-nitroso-N-acetylpenicillamine (SNAP), a donor of NO. Additionally, glibenclamide, a blocker of the KATP channel, and sildenafil, a phosphodiesterase inhibitor, were also administered. Results: The results showed that intraperitoneal injection of polydatin at different doses (5, 10, and 15 mg/kg) led to dose-dependent and significant anti-nociception in both the early and late phases of the formalin test. The anti-nociceptive effects of polydatin were augmented when mice were pretreated with SNAP, while pretreatment with L-NAME and glibenclamide reduced these effects in the formalin test. Conclusions: In conclusion, this study demonstrates the anti-nociceptive effect of polydatin and the involvement of the NO/cGMP/KATP channel pathway in such an effect. | en |
| DOI | https://doi.org/10.5812/jjnpp-163665 | en |
| Keyword | Polydatin | en |
| Keyword | Nociceptive Pain | en |
| Keyword | Formalin Test | en |
| Keyword | cGMP | en |
| Keyword | L-arginine | en |
| Keyword | Nitric Oxide | en |
| Keyword | Potassium Channel | en |
| Publisher | Brieflands | en |
| Title | Polydatin Relieves Nociceptive Pain: Possible Role of L-arginine/NO/cGMP/K<sub>ATP</sub> Channel Signaling Pathway in Mice | en |
| Type | Research Article | en |