Combination of Ethanolic Extract of Citrus aurantifolia Peels with Doxorubicin Modulate Cell Cycle and Increase Apoptosis Induction on MCF-7 Cells
| Author | Anugerah Budipratama Adina | en |
| Author | Fina Aryani Goenadi | en |
| Author | Franciscus Feby Handoko | en |
| Author | Dwi Ana Nawangsari | en |
| Author | Adam Hermawan | en |
| Author | Riris Istighfari Jenie | en |
| Author | Edy Meiyanto | en |
| Issued Date | 2014-07-31 | en |
| Abstract | New approach of breast cancer therapy is developed toward combination therapy with agents that have a specific molecular target. Our previous study showed that Citrus aurantifolia lime peels ethanolic extract (CPE) increased the sensitivity of MCF-7 cells againts doxorubicin. This study aims to observe the mechanism of combination CPE and doxorubicin in cell cycle modulation and apoptosis on MCF-7 cells. The assays were performed in the study were cell cycle assay, apoptosis induction, and immunocytochemistry of MCF-7 cells.The effect on the modulation of cell cycle and apoptosis were observed by flowcytometry assay in both single dose of CPE and its combination with Doxorubicin. Cell cycle distribution were observed with flowcytometer FACS-Calibur and its data was analyzed by Cell Quest program. Apoptotic induction in MCF-7 cells was examined using acrydine orange-ethidium bromide (AO-EtBr) double staining. Immunocytochemistry assay was done to observe the expression of apoptotic regulation protein p53 and Bcl-2. | en |
| DOI | https://doi.org/10.22037/ijpr.2014.1551 | en |
| Keyword | <i>Citrus aurantifolia </i>peels | en |
| Keyword | Doxorubicin | en |
| Keyword | MCF-7 | en |
| Keyword | Cell cycle | en |
| Keyword | Apoptosis | en |
| Publisher | Brieflands | en |
| Title | Combination of Ethanolic Extract of Citrus aurantifolia Peels with Doxorubicin Modulate Cell Cycle and Increase Apoptosis Induction on MCF-7 Cells | en |
| Type | Original Article | en |