Identification of DR75-RS11090 Gene Encoding GGDEF Domains, and its Role in Enterococcus faecalis
| Author | Luna Fan | en |
| Author | Benxiang Hou | en |
| Issued Date | 2019-01-31 | en |
| Abstract | Background: Enterococcus faecalis is considered the primary pathogenic bacteria in failed root canal therapy due to its excellent biofilm formation ability. The second messenger cyclic dimeric (3’→5’) GMP (c-di-GMP) is involved in bacterial physiological processes, including biofilm formation. C-di-GMP is formed by diguanylate cyclases with the GGDEF domain. Objectives: The current study aimed at investigating the function of DR75-RS11090 in E. faecalis. Methods: In the current experimental study, gene encoding GGDEF domains in E. faecalis were analyzed through National Center for Biotechnology Information (NCBI) database. Enterococcus faecalis strains were cultured in brain-heart-infusion (BHI) broth at 37°C. Growth curves of E. faecalis were measured in an automatic microplate reader. Biofilms were fixed with 2% glutaraldehyde and scanned under the transmission electron microscopy. Acid and water-insoluble polysaccharide productions were also detected by the precise digital acidometer and anthrone method. Results: It was found that DR75-RS11090 gene was predicted to encode GGDEF domain in E. faecalis. The current study observed promoting effects of DR75-RS11090 on E. faecalis biofilm formation, growth, acid production, and water insoluble polysaccharide production. Conclusions: The current study findings improved the understanding of c-di-GMP in E. faecalis and presented a primary study on DR75-RS11090 promoting mechanism. | en |
| DOI | https://doi.org/10.5812/jjm.83394 | en |
| URI | https://brieflands.com/journals/jjm/articles/83394 | en |
| Keyword | Biofilms | en |
| Keyword | c-di-GMP | en |
| Keyword | <i>Enterococcus faecalis</i> | en |
| Keyword | DR75-RS11090 | en |
| Keyword | GGDEF | en |
| Publisher | Brieflands | en |
| Title | Identification of DR75-RS11090 Gene Encoding GGDEF Domains, and its Role in <i>Enterococcus faecalis</i> | en |
| Type | Research Article | en |