A Simple Sample Preparation with HPLC-UV Method for Estimation of Clomipramine from Plasma

Abstract

Clomipramine is a tricyclic antidepressant. Different methods for determination of clomipramine hydrochloride in plasma have been described. Most of these procedures favor the use of acidic back-extraction in extraction procedure and HPLC as the analytical technique. In this study, the clomipramine extraction procedure was modified and a direct injection to the column was performed to shorten the time of sample preparation considerably. Furthermore, the method was applied in bioequivalence study of new formulations of clomipramine in comparison with reference tablets.