Hepatitis C virus (HCV) Infection Rate among Seronegative Hemodialysis Patients Screened by Two Methods; HCV Core Antigen and Polymerase Chain Reaction
| Author | Maryam Moini | en |
| Author | Mazyar Ziyaeyan | en |
| Author | Shapoor Aghaei | en |
| Author | Mohammad Mahdi Sagheb | en |
| Author | Seyed Alireza Taghavi | en |
| Author | Mahsa Moeini | en |
| Author | Marzieh Jamalidoust | en |
| Author | Laleh Hamidpour | en |
| Issued Date | 2013-05-01 | en |
| Abstract | Background: End-stage renal disease patients on chronic hemodialysis are among high risk groups for hepatitis C virus (HCV) infection for whom routine HCV screening is recommended. Anti-HCV antibody (ab) testing may not be reliable to detect all infected cases because of the blunted ab response due to depressed immune state in these patients. Using a more reliable, cost-effective and non-complex HCV screening test may be necessary in this group of patients for case finding and management, and also for prevention of infection spread. Objectives: The aim of this study was to find the prevalence of HCV infection in HCV ab negative hemodialysis patients by Real time PCR and total HCV core antigen (ag) test and comparing the results of the two tests. Patients and Methods: From a single hemodialysis center, 181 anti- HCV ab negative patients were screened by total HCV core ag using an ELISA kit. Real time PCR was used for determination of the virus and viral load quantity. Results: Among the 181 anti-HCV ab negative patients, 13 (7.2%) were positive for HCV core ag and 11 (6%) had detectable HCV RNA with a range of 40-336543 IU/ml by PCR. The two tests had a high measurement agreement (Kappa=0.82, P Discussion: The rate of HCV infection among HCV ab negative hemodialysis patients was high. HCV core ag testing could be used as a sensitive method for HCV infection screening in this group of patients. | en |
| DOI | https://doi.org/10.5812/hepatmon.9147 | en |
| Keyword | Hepatitis C Virus | en |
| Keyword | Core Antigen | en |
| Keyword | Hemodialysis | en |
| Keyword | Polymerase Chain Reaction | en |
| Publisher | Brieflands | en |
| Title | Hepatitis C virus (HCV) Infection Rate among Seronegative Hemodialysis Patients Screened by Two Methods; HCV Core Antigen and Polymerase Chain Reaction | en |
| Type | Research Article | en |
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