Effects of Pinoresinol from Vietnamese <i>Gnetum montanum</i> Markgr Inhibits the Inflammation in Macrophages
| Author | Cuong Trinh Tat | en |
| Author | Duong Duc Thien | en |
| Author | Nguyen Thi Hanh Tran | en |
| Author | Nguyen Anh Duc | en |
| Author | Hoang Duc Manh | en |
| Author | Nguyen Quang Huy | en |
| Author | Ly Huyen Ngo | en |
| Author | Nguyen Quang Nguyen | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: Pinoresinol, an ingredient in Gnetum montanum Markgr, has been demonstrated to be an anti-inflammatory substance. However, the anti-inflammatory activity of pinoresinol requires further research. Objectives: This study elucidates the effects of pinoresinol from Vietnamese G. montanum Markgr in suppressing lipopolysaccharide (LPS)-stimulated inflammation and the production of reactive oxygen species (ROS) in macrophages. Methods: Methanol was used to extract pinoresinol from Vietnamese G. montanum Markgr. The purity of pinoresinol was evaluated by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 was analyzed by Western blot assay. Immunofluorescence was used to measure ROS generated from Raw 264.7 cells. Results: Lipopolysaccharide-mediated secretion of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β was significantly decreased by pinoresinol (40 µg/mL, P < 0.001). Furthermore, LPS-induced phosphorylation of ERK1/2 and p38 was markedly suppressed by pinoresinol (40 µg/mL, P < 0.001). Importantly, ROS generated by LPS in macrophages was inhibited by pinoresinol at a concentration of 40 µg/mL (P < 0.001). Conclusions: These data further elucidate the anti-inflammatory pathways of pinoresinol, targeting the mitogen-activated protein kinase (MAPK) signaling pathways and ROS. | en |
| DOI | https://doi.org/10.5812/jrps-156394 | en |
| Keyword | <i>Gnetum montanum</i> Markgr | en |
| Keyword | Inflammation | en |
| Keyword | Pinoresinol | en |
| Keyword | Reactive Oxygen Species | en |
| Publisher | Brieflands | en |
| Title | Effects of Pinoresinol from Vietnamese <i>Gnetum montanum</i> Markgr Inhibits the Inflammation in Macrophages | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jrps-13-1-156394-publish-pdf.pdf
- Size:
- 951.48 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF