Antioxidant and Protective Effects of Angipars™, a New Herbal Drug, on Hydrogen Peroxide-Induced Oxidative Stress in HepG<sub>2</sub> and A549 Cell Lines
| Author | Elina Asiaei | en |
| Author | Melika Farshad | en |
| Author | Mohammad Abdollahi | en |
| Author | Akram Jamshidzadeh | en |
| Author | Amin Reza Akbarizadeh | en |
| Author | Mohammad Amin Soltanzadeh | en |
| Issued Date | 2016-11-30 | en |
| Abstract | In the present study, free-radical-scavenging and antioxidant activities of Angipars™, an herbal extract which has been registered as a therapeutic drug for diabetic foot ulcer, were examined. We investigated the protective effect of Angipars™ on cytotoxicity produced by exposure to H2O2 (300 µM) in HepG2 and A549 cell lines, using MTT assay, glutathione (GSH) content analysis, and lipid peroxidation evaluation. Angipars™ concentrations of 1 - 20 µg/mL effectively scavenged DPPH radicals in a dose-dependent manner, while hydroxyl-radical-scavenging activity was only observed at high doses. Following a 4-hour cell treatment with H2O2, a noticeable decline in cell viability was observed, which was alleviated by Angipars™ (pre-incubated for 24 hours) at high doses. Angipars™ significantly reduced GSH content and products of lipid peroxidation, resulting from H2O2 exposure. These findings suggest that Angipars™ exhibits positive antioxidant activity in vitro and can attenuate H2O2-induced cytotoxicity; in fact, this may represent the cellular mechanisms for its cytoprotective activity. | en |
| DOI | https://doi.org/10.17795/jjnpp-42944 | en |
| Keyword | Angipars | en |
| Keyword | Oxidative Stress | en |
| Keyword | Antioxidant | en |
| Keyword | Cytoprotective | en |
| Publisher | Brieflands | en |
| Title | Antioxidant and Protective Effects of Angipars™, a New Herbal Drug, on Hydrogen Peroxide-Induced Oxidative Stress in HepG<sub>2</sub> and A549 Cell Lines | en |
| Type | Research Article | en |
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