First Transcriptome Analysis of Iranian Scorpion, <i>Mesobuthus Eupeus </i>Venom Gland

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AuthorMasoumeh Baradaranen
AuthorAmir Jalalien
AuthorMaryam Naderi-Soorkien
AuthorMahmoud Jokaren
AuthorHamid Galehdarien
Issued Date2018-10-31en
AbstractScorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyzed the transcriptome based on expressed sequence tag (EST) database from the venom tissue of Iranian M. eupeus by constructing a cDNA library and subsequent Sanger sequencing of obtained inserts. Sixty-three unique transcripts were identified, which were grouped in different categories, including Toxins (44 transcripts), Cell Proteins (9 transcripts), Antimicrobial Peptides (4 transcripts) and Unknown Peptides (3 transcripts). The analysis of the ESTs revealed several new components categorized among various toxin families with effect on ion channels. Sequence analysis of a new precursor provides evidence to validate the first CaTxs from M. eupeus. The results are exploration of the diversity of precursors expressed of Iranian M. eupeus venom gland. We further described comparative analysis of venom components of Iranian M. eupeus with other sibling species.en
DOIhttps://doi.org/10.22037/ijpr.2018.2300en
KeywordcDNA libraryen
Keyword<i>Mesobuthus eupeus</i>en
KeywordTranscriptome analysisen
KeywordChlorotoxin-like peptideen
KeywordHomology modelingen
KeywordMolecular dynamics flexible fitting (MDFF)en
PublisherBrieflandsen
TitleFirst Transcriptome Analysis of Iranian Scorpion, <i>Mesobuthus Eupeus </i>Venom Glanden
TypeOriginal Articleen

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Iranian Journal of Pharmaceutical Research