Protective Effect of Dizocilpine (MK-801) On TNBS-Induced Experimental Colitis in Mice
| Author | Ehsan Motaghi | en |
| Author | Valiollah Hajhashemi | en |
| Author | Parvin Mahzouni | en |
| Author | Mohsen Minaiyan | en |
| Issued Date | 2019-07-31 | en |
| Abstract | Ulcerative colitis is chronic and recurrent disease of the gastrointestinal tract with uncertain etiology and incomplete treatment options. N-methyl-d-aspartate (NMDA) receptor suppression has shown anti-inflammatory effects in-vitro and in-vivo. The aim of present study was to evaluate the role of dizocilpine (MK-801), a noncompetitive NMDA receptor antagonist, on TNBS (trinitrobenzene sulfonic acid)-induced murine model of colitis. Dizocilpine (0.1, 1 and 5 mg/kg) was given to mice intraperitoneally from 24 h before induction of colitis and daily thereafter for 4 days. Dexamethasone (1 mg/kg) was used as the reference drug. Colitis was induced by intracolonic administration of TNBS/Ethanol (50/50 v/v, 40mg/kg). Animals were sacrificed 5 days after colitis induction and distal colons were examined macroscopically and microscopically. The colonic tissue level of pro-inflammatory cytokines including interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α) were assessed by ELISA. Myeloperoxidase (MPO) level was also measured in colon. Dizocilpine, particularly with intermediate dose of 1mg/kg significantly improved animal’s weight loss as well as macroscopic and microscopic signs of colitis, reduced colonic levels of IL-1β, IL-6, TNF-α and MPO activity. Hence, dizocilpine has significant protective effects in TNBS-induced colitis and NMDA suppression may be a new and effective therapeutic strategy in ulcerative colitis via decreasing in pro-inflammatory cytokine production. | en |
| DOI | https://doi.org/10.22037/ijpr.2019.1100751 | en |
| Keyword | Dizocilpine | en |
| Keyword | MK-801 | en |
| Keyword | Ulcerative colitis | en |
| Keyword | Mice | en |
| Keyword | NMDA | en |
| Publisher | Brieflands | en |
| Title | Protective Effect of Dizocilpine (MK-801) On TNBS-Induced Experimental Colitis in Mice | en |
| Type | Original Article | en |