The First Report of CMY, aac (6′)-Ib and 16S rRNA Methylase Genes Among Pseudomonas aeruginosa Isolates From Iran
| Author | Mohsen Jafari | en |
| Author | Fatemeh Fallah | en |
| Author | Rebwar Shams Borhan | en |
| Author | Masoumeh Navidinia | en |
| Author | Abdollah Karimi | en |
| Author | Sedigheh Rafiei Tabatabaei | en |
| Author | Ali Hashemi | en |
| Orcid | Ali Hashemi [0000-0002-7258-5541] | en |
| Issued Date | 2013-10-01 | en |
| Abstract | Background:: Serious infections by Pseudomonas aeruginosa are commonly treated with the combination of a beta-lactam antibacterial and an aminoglycoside. Therefore, production of a 16S rRNA methylase may result in an extremely important antibacterial resistance profile. Objectives:: The present study was conducted to determine the prevalence of Cephamycinase (CMY), aminoglycoside 6'-N-acetyltransferase (aac(6′)-Ib) and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran. Patients and Materials:: This descriptive study was performed on hospitalized burnt patients during 2011and 2012. Antibiotics susceptibility tests were performed by disc diffusion and broth microdilution methods. CMY, aac(6′)-Ib, 16S rRNA methylase genes were detected by PCR method. Results:: Seventy-seven (77%) of 100 isolates were resistant to Imipenem and Ceftazidime. aac(6)-Ib, Cephamycinase (CMY), and rRNA methyltransferase (rmtB and rmtD) were detected in 57 (74.02%), 7 (9.09%), 11 (14.28%), and 9 (11.68%) isolates, respectively. PCR results for aminoglycoside resistance methyltransferase (armA) and rRNA methyltransferase (rmtC) were all negative. Aminoglycoside resistance methyltransferase (armA), and rRNA methyltransferase (rmtC) were not detected. Conclusions:: This study detected multiple drug resistance in Pseudomonas aeruginosa including resistance to β-lactams, Aminoglycosides, and Fluoroquinolones. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward. | en |
| DOI | https://doi.org/10.5812/pedinfect.11392 | en |
| Keyword | Pseudomonas aeruginosa | en |
| Keyword | Drug Resistance | en |
| Keyword | Multiple | en |
| Keyword | Bacterial | en |
| Keyword | Burn Units | en |
| Publisher | Brieflands | en |
| Title | The First Report of CMY, aac (6′)-Ib and 16S rRNA Methylase Genes Among Pseudomonas aeruginosa Isolates From Iran | en |
| Type | Research Article | en |
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