Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology
| Author | Mohammad Hossein Morowvat | en |
| Author | Valiollah Babaeipour | en |
| Author | Hamid Rajabi Memari | en |
| Author | Hossein Vahidi | en |
| Issued Date | 2015-04-01 | en |
| Abstract | Background: The periplasmic overexpression of recombinant human interferon beta (rhIFN-β)-1b using a synthetic gene in Escherichia coli BL21 (DE3) was optimized in shake flasks using Response Surface Methodology (RSM) based on the Box-Behnken Design (BBD). Objectives: This study aimed to predict and develop the optimal fermentation conditions for periplasmic expression of rhIFN-β-1b in shake flasks whilst keeping the acetate excretion as the lowest amount and exploit the best results condition for rhIFN-β in a bench top bioreactor. Materials and Methods: The process variables studied were the concentration of glucose as carbon source, cell density prior the induction (OD 600 nm) and induction temperature. Ultimately, a three-factor three-level BBD was employed during the optimization process. The rhIFN-β production and the acetate excretion served as the evaluated responses. Results: The proposed optimum fermentation condition consisted of 7.81 g L-1 glucose, OD 600 nm prior induction 1.66 and induction temperature of 30.27°C. The model prediction of 0.267 g L-1 of rhIFN-β and 0.961 g L-1 of acetate at the optimum conditions was verified experimentally as 0.255 g L-1 and 0.981 g L-1 of acetate. This agreement between the predicted and observed values confirmed the precision of the applied method to predict the optimum conditions. Conclusions: It can be concluded that the RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli. | en |
| DOI | https://doi.org/10.5812/jjm.8(4)2015.16236 | en |
| URI | https://brieflands.com/journals/jjm/articles/56382 | en |
| Keyword | Periplasmic | en |
| Keyword | Acetic acid | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | Interferon-beta | en |
| Publisher | Brieflands | en |
| Title | Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology | en |
| Type | Research Article | en |
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