A Simple Method for Extraction and Purification of Hypericins from St John’s Wort
| Author | Zahra Ramezani | en |
| Author | Mitra Zamani | en |
| Issued Date | 2017-02-28 | en |
| Abstract | Hypericin was extracted from Hypericum perforatum L. using a different extraction procedure. Highest amounts of hypericins were extracted by ultrasonic methods using methanol: acetone 2:1 as an extracting solvent. Hypericin contents of the extract was purified by passing it through 2 series of 70 × 5 mm glass columns, each filled with 800 mg of 35 - 70 mesh silica gel, followed by elution with MeOH: acetone: CH2Cl2 (75:10:15). The extent of purification was determined by HPLC. The amount of purified hypericins, which was extracted from Hypericum perforatum, was 5.105 mg per gram dried plant leaves. | en |
| DOI | https://doi.org/10.5812/jjnpp.13864 | en |
| Keyword | Hypericin | en |
| Keyword | Pseudo-hypericin | en |
| Keyword | HPLC | en |
| Keyword | Ultrasonic Assisted Extraction | en |
| Keyword | <i>Hypericum perforatum L.</i> | en |
| Publisher | Brieflands | en |
| Title | A Simple Method for Extraction and Purification of Hypericins from St John’s Wort | en |
| Type | Brief Report | en |