Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes
| Author | Shabnam Sadoogh Abbasian | en |
| Author | Ehsanollah Ghaznavi Rad | en |
| Author | Neda Akbari | en |
| Author | Mohammad Reza Zolfaghari | en |
| Author | Iraj pakzad | en |
| Author | Hamid Abtahi | en |
| Orcid | Hamid Abtahi [0000-0002-3247-3289] | en |
| Issued Date | 2015-01-01 | en |
| Abstract | Background: Hyaluronidase catalyzes the hydrolysis of hyaluronan polymers to N-acetyl-D-glucosamine and D-glucuronic acid. This enzyme is a dimer of identical subunits. Hyaluronidase has different pharmaceutical and medical applications. Previously, we produced a recombinant hyaluronidase antigenic fragment of Streptococcus pyogenes. Objectives: This study aimed to improve the protein production and purity of hyaluronidase recombinant protein from S. pyogenes. In addition, the enzymatic activity of this protein was investigated. Materials and Methods: The expression of hyaluronidase antigenic fragments was optimized using IPTG concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. Afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. Results: Data indicated that maximum protein is produced in OD = 0.8, 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG), 37ºC, NB 1.5x, without glucose, incubated for overnight. The enzymatic activity of the recombinant protein was similar to the commercial form of hyaluronidase. Conclusions: The results showed that an antigenic fragment of the recombinant hyaluronidase protein from S. pyogenes has a considerable enzymatic activity. It can be suggested to use it for medical purposes. In addition, applications of bioinformatics software would facilitate the production of a smaller protein with same antigenic properties and enzymatic activity. | en |
| DOI | https://doi.org/10.5812/jjm.13653 | en |
| Keyword | Hyaluronidase | en |
| Keyword | Optimization of Expression | en |
| Keyword | Enzymatic Activity | en |
| Publisher | Brieflands | en |
| Title | Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes | en |
| Type | Research Article | en |
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