Antiproliferative and Pro-apoptotic Activities of <i>Tournefortia mutabilis</i> vent. Leaves on the Human Breast Adenocarcinoma Cell Line (MCF-7)
| Author | Zoila Mora-Guzmán | en |
| Author | Luis Bernardo Flores-Cotera | en |
| Author | Eduardo Pérez-Campos | en |
| Author | Rebeca López-Marure | en |
| Author | Delia Soto-Castro | en |
| Author | Felipe Alonso Masso-Rojas | en |
| Author | Araceli Paéz Arenas | en |
| Author | Edgar Zenteno | en |
| Author | Margarito Martinez-Cruz | en |
| Author | Laura Pérez-Campos Mayoral | en |
| Author | María Teresa Hernández-Huerta | en |
| Author | María del Socorro Pina-Canseco | en |
| Orcid | María Teresa Hernández-Huerta [0000-0003-2182-2540] | en |
| Orcid | María del Socorro Pina-Canseco [0000-0002-9486-5093] | en |
| Issued Date | 2024-12-31 | en |
| Abstract | Background: Breast cancer is the most common cancer among women worldwide, impacting not only the patients but also their families and communities. Tournefortia mutabilis vent. is a plant endemic to Mexico, traditionally used in Zapotec medicine for the treatment of cancer. Objectives: This study aims to evaluate the effects of the chloroformic extract of T. mutabilis vent. leaves on cell proliferation and cell death in MCF-7 cells. Methods: The effect of the extract on MCF-7 cell proliferation was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet staining. Apoptosis was evaluated through fluorescein diacetate/propidium iodide staining and caspase-3, -6, and -9 activity assays. Results: The half-maximal inhibitory concentration (IC50) of the T. mutabilis vent. extract on MCF-7 cell proliferation at 48 hours and 72 hours after treatment was 86.4 µg/mL and 2.74 µg/mL, respectively. We observed that the extract and its semi-purified fractions induced cell death through the activation of caspases 3, -6, and -9. Conclusions: Tournefortia mutabilis vent. is a potential source of compounds with antiproliferative and pro-apoptotic activities on the MCF-7 cell line, primarily through the intrinsic pathways of apoptosis. | en |
| DOI | https://doi.org/10.5812/ijpr-149405 | en |
| URI | https://brieflands.com/journals/ijpr/articles/149405 | en |
| Keyword | Apoptosis | en |
| Keyword | Medicinal Plants | en |
| Keyword | Anticancer | en |
| Keyword | Pro-apoptotic | en |
| Keyword | Breast Neoplasms | en |
| Keyword | MCF-7 Cells | en |
| Publisher | Brieflands | en |
| Title | Antiproliferative and Pro-apoptotic Activities of <i>Tournefortia mutabilis</i> vent. Leaves on the Human Breast Adenocarcinoma Cell Line (MCF-7) | en |
| Type | Research Article | en |