Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic <italic>Bacteroides fragilis</italic> Isolated From Stools
| Author | Mohammad Taghi Akhi | en |
| Author | Reza Ghotaslou | en |
| Author | Mahnaz Shirinzadeh | en |
| Author | Tahereh Pirzadeh | en |
| Author | Mohammad Naghavi Behzad | en |
| Issued Date | 2013-06-30 | en |
| Abstract | Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria whichcause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobicbacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis. Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency thedisk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates. Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods. Results: Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofloxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, while the lowest resistance in two methods was obtained for imipenem, piperacillin / tazobactam, and metronidazol. 100% agreement for some of antibiotics such as imipenem (P ? 0.05), and no correlation for others were observed among the antimicrobial susceptibility results obtained by the two methods (P ? 0.05). Conclusions: The presence of bft gene in B. fragilis isolates wouldnot certainly result in diarrheaamong patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed. | en |
| DOI | https://doi.org/10.5812/jjm.9800 | en |
| Keyword | <italic>Bacteroides fragilis</italic> | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | Disk Diffusion Antimicrobial Tests | en |
| Publisher | Brieflands | en |
| Title | Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic <italic>Bacteroides fragilis</italic> Isolated From Stools | en |
| Type | Research Article | en |
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