Simple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
| Author | Mohsen Amini | en |
| Author | Mahnaz Khanavi | en |
| Author | Abbas Shafiee | en |
| Issued Date | 2004-04-30 | en |
| Abstract | A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-liquid extraction and the average recovery was 78.2% The assay is precise, with inter-assay coefficient of variation of 6.70 % at 0.25-8 µg/ml (n=3). Using UV detection at 277 nm the detection limit for ciprofloxacin was 20 ng/ml of plasma and the mean extraction recovery was 78.2 %. Short elution time, using UV detector and usage of ofloxacin as internal standard are advantages of this method. | en |
| DOI | https://doi.org/10.22037/ijpr.2010.583 | en |
| Keyword | HPLC | en |
| Keyword | Plasma | en |
| Keyword | Determination | en |
| Keyword | Ciprofloxacin | en |
| Publisher | Brieflands | en |
| Title | Simple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma | en |
| Type | Research Article | en |
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