A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats
| Author | Marjan Daeihamed | en |
| Author | Azadeh Haeri | en |
| Author | Simin Dadashzadeh | en |
| Issued Date | 2015-03-31 | en |
| Abstract | A validated HPLC method was developed to determine the doxorubicin concentration in a small volume of rat plasma (60 µL) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using a minimum amount of organic solvent, with extraction recovery more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mobile phase consisted of acetonitrile and water (32:68, v/v; pH=2.6). The flow-rate was kept at 1 mL/min and the column effluent was monitored with a fluorescence detector at an excitation and emission wavelength of 470 and 555 nm, respectively. The detection limit was 5 ng/mL. No analytical interference was observed from endogenous components in the rat plasma. This method was feasibly applied to the pharmacokinetic study of 5 mg/Kg of doxorubicin after the intravenous administration to rats. | en |
| DOI | https://doi.org/10.22037/ijpr.2015.1710 | en |
| Keyword | Doxorubicin | en |
| Keyword | HPLC | en |
| Keyword | Fluorescence | en |
| Keyword | Pharmacokinetics | en |
| Keyword | Rat plasma | en |
| Keyword | Small sample volume | en |
| Publisher | Brieflands | en |
| Title | A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats | en |
| Type | Original Article | en |
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