Electrospun PVA Nanofibers Loaded with Antimicrobial Herbal Extracts for Healing the Infectious Wound
| Author | Parisa Gheibi | en |
| Author | Nazanin Jabbari | en |
| Author | Nazanin Kafi Alghari | en |
| Author | Sabina Mah Nesaei | en |
| Author | Ramin Farhoudi | en |
| Author | Zohre Eftekhari | en |
| Orcid | Parisa Gheibi [0000-0002-0645-317X] | en |
| Orcid | Nazanin Jabbari [0000-0002-2926-9631] | en |
| Orcid | Zohre Eftekhari [0000-0003-3502-9260] | en |
| Issued Date | 2024-02-29 | en |
| Abstract | Background: Low-cost, soluble polyvinyl alcohol (PVA) polymers enhance the efficacy of herbal formulations with established antimicrobial properties. Objectives: This study investigates the healing process of Staphylococcus aureus-infected wounds using PVA loaded with herbal extracts, including Arnebia euchroma, Allium sativum, and Echinacea purpurea. Methods: minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) assays, Disk Diffusion Method (DDM) tests, and Gas Chromatography-Mass Spectrometry (GC/MS) analyses were conducted on herbal extract samples. Twelve male Wistar rats were divided into G1: Negative control group (healthy mice), G2: Wound model + S. aureus (Positive control), G3: wound model + S. aureus + Povidone-iodine, and G4: Wound model + S. aureus + PVA/formulation. Hematoxylin-eosin and immunofluorescent staining were employed to assess wound healing. Results: The ethanolic herbal extract exhibited potent antibacterial activity against S. aureus, with MIC and MBC values in the 1.87 mg/mL range. The PVA/formulation displayed a zone of inhibition with a diameter of 7 mm against S. aureus. Histopathological investigations indicated that the infected wound treated with nanofibers extract experienced a significant reduction in inflammation between days 7 and 14. Notably, the increased CD3 expression at this site was remarkable for the healing process. Conclusions: Consequently, this experimental study suggests combining PVA and herbal extracts enhances antibacterial properties and promotes CD3 expression and re-epithelialization effects. | en |
| DOI | https://doi.org/10.5812/jjnpp-137995 | en |
| Keyword | Antimicrobial | en |
| Keyword | Herbal Medicine | en |
| Keyword | Nanofiber | en |
| Keyword | <i>Staphylococcus aureus</i> | en |
| Publisher | Brieflands | en |
| Title | Electrospun PVA Nanofibers Loaded with Antimicrobial Herbal Extracts for Healing the Infectious Wound | en |
| Type | Research Article | en |