Efficacy of Phage-Antibiotic Combinations Against Multidrug-Resistant <i>Klebsiella pneumoniae</i> Clinical Isolates
| Author | Hafiza Qurat-ul-Ain | en |
| Author | Muhammad Ijaz | en |
| Author | Abu Baker Siddique | en |
| Author | Saima Muzammil | en |
| Author | Muhammad Shafique | en |
| Author | Muhammad Hidayat Rasool | en |
| Author | Ahmad Almatroudi | en |
| Author | Mohsin Khurshid | en |
| Author | Tamoor Hamid Chaudhry | en |
| Author | Bilal Aslam | en |
| Orcid | Abu Baker Siddique [0000-0002-8589-2292] | en |
| Orcid | Saima Muzammil [0000-0002-7775-8878] | en |
| Orcid | Ahmad Almatroudi [0000-0002-1491-6402] | en |
| Orcid | Mohsin Khurshid [0000-0002-3196-2857] | en |
| Orcid | Bilal Aslam [0000-0002-5568-5049] | en |
| Issued Date | 2021-01-31 | en |
| Abstract | Background: Increasing antibiotic resistance warrants therapeutic alternatives to eradicate resistant bacteria. Combined phage-antibiotic therapy is a promising approach for eliminating bacterial infections and limiting the evolution of therapy-resistant diseases. Objectives: In the present study, we evaluated the effects of combinations of bacteriophages and antibiotics against multidrug-resistant (MDR) Klebsiella pneumoniae. Methods: Two MDR strains (GenBank no. MF953600 & MF953599) of K. pneumoniae were used. Bacteriophages were isolated from hospital sewage samples by employing a double agar overlay assay and identified by transmission electron microscopy. For further characterization of bacteriophages, the killing assay and host range test were performed. To assess therapeutic efficacy, phages (7.5 × 104 PFU/mL) were used in combination with various antibiotics. Results: The phage-cefepime and tetracycline combinations displayed promising therapeutic effects, restricting the growth of K. pneumoniae isolates, as evidenced by recording OD650nm values. Conclusions: The results of the current study showed that phage-antibiotic combination was a potential therapeutic approach to treat the infections caused by MDR K. pneumoniae. | en |
| DOI | https://doi.org/10.5812/jjm.111926 | en |
| Keyword | Bacteriophages | en |
| Keyword | <i>Klebsiella pneumonia</i> | en |
| Keyword | Tetracycline | en |
| Keyword | ESKAPE | en |
| Keyword | MDR | en |
| Publisher | Brieflands | en |
| Title | Efficacy of Phage-Antibiotic Combinations Against Multidrug-Resistant <i>Klebsiella pneumoniae</i> Clinical Isolates | en |
| Type | Research Article | en |