The Synergistic Antibacterial Effect of Topical Phage Cocktail and Gentamicin on the Healing Process of Infectious Wounds by Klebsiella pneumoniae in Wistar Rats Model
| Author | Hussein T. Abdulabbas | en |
| Author | Jihan Fadhil Ashraf | en |
| Author | Saja Mahdey Jaber | en |
| Author | Ali H. Al-Marzoqi | en |
| Author | Ahmed Al-Shammari | en |
| Author | Saade Abdalkareem Jasim | en |
| Author | Sundus Jassim Muhammad | en |
| Author | Raed Obaid Saleh | en |
| Author | Mohsen Karami | en |
| Author | Niloofar Rahimian | en |
| Author | Kazem Javanmardi | en |
| Author | Ali Ojaghverdizadeh | en |
| Author | Javad Jokar | en |
| Orcid | Niloofar Rahimian [0009-0007-5400-0363] | en |
| Orcid | Kazem Javanmardi [0000-0003-0485-6722] | en |
| Orcid | Javad Jokar [0009-0007-2100-8190] | en |
| Issued Date | 2026-06-30 | en |
| Abstract | Background: Infections caused by drug-resistant Klebsiella pneumoniae represent a significant and growing global problem. Bacteriophages are viruses that target specific bacteria and represent a promising alternative to antibiotics for eradicating bacterial infections. Objectives: This study investigated the therapeutic effect of a phage cocktail on wounds infected with clinical and standard K. pneumoniae (ATCC® BAA-1705) strains. Methods: Specific bacteriophages were isolated from hospital sewage samples. An infectious wound model was established in Wistar rats, and the effects of a hydroxypropyl methylcellulose (HPMC) gel containing a phage cocktail were evaluated in animals with infected wounds compared with gentamicin. Finally, the antibacterial and wound-healing effects of combination therapy (HPMC gel containing phage cocktail + gentamicin) were investigated in vivo. Results: The results revealed that a single dose of a phage cocktail significantly reduced the mortality rate in rats with K. pneumoniae wound infection. We also observed that combination therapy (phage cocktail + gentamicin) significantly accelerated infected wound healing and reduced the number of bacterial colonies and the mortality rate in rats. Conclusions: Complementary therapy using a phage cocktail in combination with antibiotics may be a promising approach for treating infected wounds. Infections caused by drug-resistant K. pneumoniae represent a significant and growing problem worldwide. | en |
| DOI | https://doi.org/10.5812/jjm-170974 | en |
| URI | https://brieflands.com/journals/jjm/articles/170974 | en |
| Keyword | Phage Therapy | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Keyword | Wounds | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | Hydroxypropyl Methylcellulose | en |
| Publisher | Brieflands | en |
| Title | The Synergistic Antibacterial Effect of Topical Phage Cocktail and Gentamicin on the Healing Process of Infectious Wounds by <i>Klebsiella pneumoniae</i> in Wistar Rats Model | en |
| Type | Research Article | en |