Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran
| Author | Farnoosh shateri | en |
| Author | Touraj Farazmandfar | en |
| Author | Ali Sharifian | en |
| Author | Reza Salehi Manzari | en |
| Author | Marzieh Attar | en |
| Author | Majid Shahbazi | en |
| Issued Date | 2017-09-30 | en |
| Abstract | Background: The Coronary artery disease (CAD) occurs as a result of atherosclerotic plaque formation. The interferon gamma (IFNγ) as a multifunctional cytokine is involved in inflammatory processes in atherosclerosis.Objectives: We investigated the relationship between IFNγ (+874T/A) SNP with CAD. Moreover, we compared IFNγ mRNA expression in CAD patients and healthy controls.Patients and Methods: This case-control study with randomized sampling included 300 patients with CAD and 301 normal controls. The SSP-PCR method was used for genotyping IFNγ (+874T/A) gene polymorphism. Quantitative Real-Time PCR was performed to measure IFNγ mRNA expression. All data was analyzed by GraphPad software. The chi-square and ordinal logistic regression tests were used to analyze differences in genotype frequencies.Results: In this study, there was a significant association between male genders with CAD (P < 0.001). There was a significant association between genotype T/T and Allele T of IFNγ (+874T/A) polymorphism with CAD (P = 0.021 and P = 0.022, respectively). The inheritance model analysis showed that two copy of allele T is required for increased risk in CAD (P = 0.031). There was a significant association between the genotype T/T of IFNγ (+874T/A) polymorphism with CAD patients with double and triple vessel disease (P = 0.030 and P = 0.013, respectively). The IFNγ mRNA level in CAD group was significantly higher than control group (P = 0.024).Conclusions: Conclusions: IFNγ gene functional polymorphism can be associated with incidence and severity of CAD. IFNγ mRNA level was also increased in CAD patients in comparison with controls. Therefore, IFNγ may play a role in predisposition to CAD. | en |
| DOI | https://doi.org/ | en |
| URI | https://brieflands.com/journals/ircrj/articles/11025 | en |
| Keyword | Interferon-Gamma | en |
| Keyword | Coronary Artery Disease | en |
| Keyword | Single Nucleotide Polymorphism | en |
| Publisher | Brieflands | en |
| Title | Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran | en |
| Type | Research Article | en |
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