Cytotoxic Activity and Phytochemical Analysis of <i>Artemisia haussknechtii</i> Boiss

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AuthorSajjad Nasserien
AuthorMohammad-Reza Delnavazien
AuthorFarshad H. Shirazien
AuthorFaraz Mojaben
OrcidSajjad Nasseri [0000-0001-5818-1324]en
OrcidFaraz Mojab [0000-0003-2415-2175]en
Issued Date2022-12-31en
AbstractCytotoxic activity of crude extract and fractions (petroleum ether, dichloromethane, and n-butanol) of Artemisia haussknechtii aerial parts was investigated by MTT assay. Dichloromethane fraction showed the highest cytotoxic effect on MCF-7 cell line (IC50 = 297.17 ± 7.99 µg/mL). Phytochemical analysis of the most effective fraction was carried out using normal phase column chromatography (CC) to get eight sub-fractions (A-H). Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for further purification. Four known compounds with cytotoxic effects on cancer cell lines were isolated from the most active fraction, including 5-Hydroxy-3',4',6,7-tetramethoxyflavone (eupatilin 7-methyl ether), 5-hydroxy 3,3',4',6,7-pentamethoxy-flavone (artemetin), 6-methoxy-7-hydroxycoumarin (scopoletin), and methyl caffeate. Structure elucidation of isolated compounds was done using spectroscopic techniques, including ESIMS and 1D-NMR (1H and 13C). Cytotoxic activity of A. haussknechtii is probably due to coumarin and flavonoid compounds.en
DOIhttps://doi.org/10.5812/ijpr-126917en
Keyword<i>Artemisia haussknechtii</i>en
KeywordMTT Assayen
KeywordPhytochemical Analysisen
Keyword5-Hydroxy-3',4',6,7-tetramethoxyflavoneen
KeywordArtemetinen
KeywordScopoletinen
KeywordMethyl Caffeateen
PublisherBrieflandsen
TitleCytotoxic Activity and Phytochemical Analysis of <i>Artemisia haussknechtii</i> Boissen
TypeResearch Articleen

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