Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli
| Author | Zahra Shoja | en |
| Author | Hamid Rajabi Memari | en |
| Author | Mohammd Roayaei Ardakani | en |
| Issued Date | 2015-08-01 | en |
| Abstract | Background: C-Phycocyanin (C-PC) from blue-green algae such as Spirulina has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-tumor activities. Recombinant β-subunit of C-PC (C-PC/β) is an inhibitor of cell proliferation and an inducer of cancer cell apoptosis. Objectives: Since C-PC/β has a big potential to be used as a promising cancer prevention or therapy agent, the purpose of this study was to clone and express Spirulina platensis cpcB gene in a bacterial expression system. This is a significant step for the production of this compound. Materials and Methods: The cpcB gene was amplified using specific primers and cloned in a bacterial expression vector, namely pET43.1a+. Gene expression of cpcB was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the dot blotting technique. Results: The SDS-PAGE analysis and dot blotting confirmed the production of recombinant C-PC/β in the bacterial expression system. Over-expression of cpcB gene was optimized in induction by 1 mM Isopropyl-β-D-Thiogalactoside (IPTG), after four hours of inoculation at 30°C. Conclusions: Over-expression of the synthetic CPC/β protein in the bacterial system (Escherichia coli BL-21) showed that E. coli can be used as a basis for further research to produce this desired protein in large quantities. | en |
| DOI | https://doi.org/10.5812/jjm.17809 | en |
| Keyword | Recombinant Proteins | en |
| Keyword | Phycocyanin | en |
| Keyword | <i>Spirulina</i> | en |
| Publisher | Brieflands | en |
| Title | Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli | en |
| Type | Research Article | en |