Rapamycin Enhances HBV Production by Inducing Cellular Autophagy
| Author | Wenjuan Huang | en |
| Author | Fengrong Zhao | en |
| Author | Ying Huang | en |
| Author | Xia Li | en |
| Author | Sufei Zhu | en |
| Author | Qin Hu | en |
| Author | Weixian Chen | en |
| Issued Date | 2014-10-01 | en |
| Abstract | Background: Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained. Objectives: In this report, we studied the mechanism by which rapamycin enhances HBV replication and expression by inducing cellular autophagy. Materials and Methods: HepG2.2.15 cells were treated with rapamycin to induce autophagy. Autophagosomes were observed by fluorescence microscopy and transmission electron microscopy. Autophagy marker protein LC3-Ⅱ/LC3-Ⅰwas detected by Western blotting. HBV DNA and mRNA were determined by real time PCR and Southern blotting. HBsAg was evaluated by ELISA. Results: In HepG2.2.15 cells, HBV DNA and HBsAg increased when host cells were treated with rapamycin and the effect was reversed by autophagy inhibitor, 3-methyladenine (3-MA). Conclusions: These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin. | en |
| DOI | https://doi.org/10.5812/hepatmon.20719 | en |
| Keyword | HBV | en |
| Keyword | Rapamycin | en |
| Keyword | Autophagy | en |
| Publisher | Brieflands | en |
| Title | Rapamycin Enhances HBV Production by Inducing Cellular Autophagy | en |
| Type | Research Article | en |