Frequency of <i>qnr </i>and <i>aac(6’)Ib-cr</i> Genes Among ESBL-Producing <i>Klebsiella pneumoniae</i> Strains Isolated from Burn Patients in Kermanshah, Iran
| Author | Siavash Vaziri | en |
| Author | Mandana Afsharian | en |
| Author | Feizollah Mansouri | en |
| Author | Mohsen Azizi | en |
| Author | Fatemeh Nouri | en |
| Author | Nahid Madadi-Goli | en |
| Author | Zainab Mohseni Afshar | en |
| Author | Mohammad Hossein Zamanian | en |
| Author | Amirhooshang Alvandi | en |
| Author | Kamal Ahmadi | en |
| Orcid | Siavash Vaziri [0000-0002-3907-6155] | en |
| Orcid | Mandana Afsharian [0000-0002-1075-4805] | en |
| Orcid | Feizollah Mansouri [0000-0002-3564-8160] | en |
| Orcid | Mohsen Azizi [0000-0002-3203-5473] | en |
| Orcid | Fatemeh Nouri [0000-0002-3879-2539] | en |
| Orcid | Nahid Madadi-Goli [0000-0002-4531-3081] | en |
| Orcid | Zainab Mohseni Afshar [0000-0002-1085-374X] | en |
| Orcid | Mohammad Hossein Zamanian [0000-0002-5459-3695] | en |
| Orcid | Amirhooshang Alvandi [0000-0002-4963-2485] | en |
| Orcid | Kamal Ahmadi [0000-0002-9830-6651] | en |
| Issued Date | 2020-07-31 | en |
| Abstract | Background: Assessment of bacteria such as Klebsiella pneumonia has shown that Plasmid-mediated quinolone resistance (PMQR) affects antibiotics resistance (e.g., quinolones). Objectives: We studied the prevalence of qnr and aac(6’)Ib-cr genes in extended-spectrum beta-lactamase (ESBL)-producing K. pneumonia strains isolated from burn wounds of patients in the city of Kermanshah, Iran. Methods: This descriptive-analytical study was conducted on 126 K. pneumonia strains isolated collected from burn wounds. Biochemical tests were used to detect the strains. The frequency of the ESBL-producing isolates was determined by phenotypic tests of the combination disk (CD) method after determining the antibiotic susceptibility pattern of the isolates through the Kirby-Bauer disc diffusion test. The prevalence of the qnr and aac(6’)-Ib-cr genes was determined using their special primers as well as polymerase chain reaction (PCR). Results: Of the 126 K. pneumonia isolates, 52 (41.3%) were identified as ESBL-producing strains. ESBL-producing isolates showed higher resistance against antibiotics than non-ESBL-producing ones. PMQR relevance and resistance to ciprofloxacin were, respectively, determined at 80.76% and 59.6%. The most frequent gene was aac(6’)-Ib-cr (n = 70, 55.6%), followed by the qnrB (n = 44, 34.9%). Conclusions: This study showed a high prevalence of qnr genes in ESBL-producing K. pneumonia isolates and antibiotic resistance. Given the horizontal transmission of antibiotic resistance genes among bacteria by mobile genetic elements, timely identification of infections caused by ESBL-producing and antimicrobial-resistant K. pneumonia strains is of paramount importance. | en |
| DOI | https://doi.org/10.5812/jjm.100348 | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Keyword | Quinolone Resistance | en |
| Keyword | Drug Resistance | en |
| Publisher | Brieflands | en |
| Title | Frequency of <i>qnr </i>and <i>aac(6’)Ib-cr</i> Genes Among ESBL-Producing <i>Klebsiella pneumoniae</i> Strains Isolated from Burn Patients in Kermanshah, Iran | en |
| Type | Research Article | en |
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