Investigation of <i>OXA-23</i>, <i>OXA-24</i>, <i>OXA-40</i>, <i>OXA-51</i>, and <i>OXA-58</i> Genes in Carbapenem-Resistant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates from Patients with Urinary Tract Infections
| Author | Elmira Pourbaghi | en |
| Author | Reza Hosseini Doust | en |
| Author | Mohammad Rahbar | en |
| Author | Marjan Rahnamaye Farzami | en |
| Orcid | Elmira Pourbaghi [0000-0003-2292-3175] | en |
| Orcid | Reza Hosseini Doust [0000-0002-9749-1250] | en |
| Orcid | Mohammad Rahbar [0000-0001-8419-3791] | en |
| Orcid | Marjan Rahnamaye Farzami [0000-0001-9745-1657] | en |
| Issued Date | 2022-02-28 | en |
| Abstract | Background: Escherichia coli and Klebsiella pneumoniae are frequently responsible for urinary tract infections (UTIs). The high rate of carbapenem resistance in Enterobacteriaceae has become a global therapeutic concern. Objectives: The study investigated OXA-23, OXA-24, OXA-40, OXA-51, and OXA-58 genes in uropathogenic E. coli and K. pneumoniae isolates. Methods: We isolated 500 uropathogenic isolates of E. coli and K. pneumoniae from patients at Milad Hospital, Tehran, Iran. Antibiotic susceptibility testing was performed using a strip-test method, and the carbapenem-nonsusceptoble isolates were confirmed with an automated antibiotic sensitivity testing system. The OXA genes were determined by multiplex PCR. Molecular typing was performed by multilocus variable-number tandem repeat (VNTR) analysis (MLVA). Results: Out of 500 isolates, 40 (8%) were detected as carbapenem-resistant, including 13 E. coli and 27 K. pneumoniae. All carbapenem-resistant isolates were ESBL-producing and resistant to ceftriaxone, ciprofloxacin, meropenem, ceftazidime, and amoxicillin-clavulanate. Moreover, 46.1% and 26% of carbapenem-insensitive E. coli and K. pneumoniae isolates carried a beta-lactamase-producing gene associated with the OXA-23-like group. Finally, E. coli and K. pneumoniae isolates were divided into two and three MLVA patterns, respectively. Conclusions: This is the first report of OXA-51, 58, and 24 carbapenemases in clinical isolates of E. coli and K. pneumoniae from UTI patients in Iran. Significant differences were seen in OXA-51, 58, and 24 genes between carbapenem-insensitive and carbapenem-sensitive E. coli and K. pneumoniae isolates. Molecular typing suggested the vertical transmission of resistance genes. | en |
| DOI | https://doi.org/10.5812/jjm-119480 | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Keyword | Carbapenem | en |
| Keyword | <i>OXA</i> Group Genes | en |
| Publisher | Brieflands | en |
| Title | Investigation of <i>OXA-23</i>, <i>OXA-24</i>, <i>OXA-40</i>, <i>OXA-51</i>, and <i>OXA-58</i> Genes in Carbapenem-Resistant <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates from Patients with Urinary Tract Infections | en |
| Type | Research Article | en |