Frequency of Extended-Spectrum Beta-Lactamases-Producing <i>Escherichia coli</i> Among Out- and In-patients in Rafsanjan City, Iran
| Author | Ebrahim Rezazadeh Zarandi | en |
| Author | Hamid Hakimi | en |
| Author | Reza Bahramabadi | en |
| Author | Mohammad Zare-Bidaki | en |
| Author | Mahnaz Ramezani | en |
| Author | Ali Shojaei | en |
| Author | Ali Khodayari | en |
| Author | Maryam Fatemipour | en |
| Author | Morteza Khademalhosseini | en |
| Author | Shokrollah Assar | en |
| Orcid | Ebrahim Rezazadeh Zarandi [0000-0001-7067-2214] | en |
| Orcid | Reza Bahramabadi [0000-0003-1920-2529] | en |
| Orcid | Morteza Khademalhosseini [0000-0002-1187-629X] | en |
| Orcid | Shokrollah Assar [0000-0001-6835-1577] | en |
| Issued Date | 2023-03-31 | en |
| Abstract | Background: The frequency of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains among clinical isolates has been steadily increasing, leading to limited treatment options. Objectives: This study aimed to assess the antibiotic sensitivity of E. coli and the frequency of ESBL isolates among both out-patients and in-patients. Methods: A total of 390 E. coli isolates were received at the Ali-Ebn-e-Abitaleb Hospital laboratory in Rafsanjan. The antibiogram, as well as the phenotypic and genotypic detection of ESBL isolates, were conducted using Kirby-Bauer, combination disk confirmatory, and polymerase chain reaction tests, respectively. Results: Of all the E. coli isolates, 45.6% exhibited ESBL production. Among these isolates, 46.1% were obtained from hospital wards, while 42.5% were from outpatients. Meropenem and imipenem displayed sensitivities of 97.2% and 93.3%, respectively, whereas amikacin and nitrofurantoin showed sensitivities of 89.7% and 85.6%, respectively, for all isolates. Notably, ceftriaxone, cefotaxime, cefixime, ampicillin, co-trimoxazole, and nalidixic acid demonstrated high resistance rates, surpassing 50%. ESBL-producing isolates were more frequently observed in blood samples (65%) and wounds (60%) compared to other tested isolates. Approximately 8.6% of isolates carried a single type of ESBL gene, while 38.5% carried multiple ESBL genes. Conclusions: The data indicate a prevalence of ESBL-producing E. coli isolates among both out-patients and in-patients, with some of them acquiring two or three types of ESBL enzymes. As a result, their ability to hydrolyze antibiotics has increased, leading to their higher occurrence in clinical samples. | en |
| DOI | https://doi.org/10.5812/jjm-133216 | en |
| Keyword | Extended-spectrum Beta-Lactamase | en |
| Keyword | Antibiotic Resistance Gene | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | In-patient | en |
| Keyword | Out-patient | en |
| Publisher | Brieflands | en |
| Title | Frequency of Extended-Spectrum Beta-Lactamases-Producing <i>Escherichia coli</i> Among Out- and In-patients in Rafsanjan City, Iran | en |
| Type | Research Article | en |
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