Evaluating the effectiveness of combination of Pure and Zeta methods in isolating mature sperms with normal chromatin structure

Abstract

Background: Isolating sperms with normal chromatin content is considered vital in Intra Cytoplasmic Sperm Injection treatment. Today, researchers are trying to find new procedures to select sperms with normal chromatin content and morphology. This study examines the effectiveness of Zeta, Density Gradient Centrifugation (Pure Sperm) and combination of Pure- Zeta procedures in selecting sperms with normal chromatin structure using Sperm Chromatin Dispersion (SCD), and Chromomycin A3 (CMA3).