Effect of Micronized Purified Flavonoid Fraction Containing Hesperidin and Diosmin on Vincristine-Induced Neuropathy in Rats; the Role of Nitric Oxide Pathway
| Author | Nikoo Abharian | en |
| Author | Nima Naderi | en |
| Author | Noushin Nikray | en |
| Author | Mona Khoramjouy | en |
| Author | Shokoofe Noori | en |
| Author | Hamed Shafaroodi | en |
| Author | Mehrdad Faizi | en |
| Orcid | Nima Naderi [0000-0003-4591-8918] | en |
| Orcid | Mona Khoramjouy [0000-0002-4607-5353] | en |
| Orcid | Mehrdad Faizi [0000-0002-6896-838X] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a potential complication that can develop following cancer chemotherapy. Objectives: Due to limited medical interventions for the prevention and management of CIPN caused by vincristine, a painful and common complication, further research to find the mechanisms of CIPN and the development of effective preventive and therapeutic strategies is needed. Methods: We induced CIPN in male Wistar rats by administering intraperitoneal vincristine (VCR) at a dose of 0.1 mg/kg for 10 days. Treatment involved micronized purified flavonoid fraction (MPFF) containing hesperidin and diosmin (MPFF; Daflon®) at doses of 50, 100, and 200 mg/kg. Our investigation focused on levels of inflammatory factors (TNF-α, IL-6) in the dorsal root ganglion (DRG) tissue and included a series of behavioral tests: Von Frey, grip strength, rotarod, open field, and hot plate tests. To examine the role of the nitric oxide (NO) pathway in the effects of MPFF on VCR-induced peripheral neuropathy (VIPN), we also tested the effect of L-arginine (100 mg/kg i.p.) as a NO precursor and L-NAME (20 mg/kg i.p.) as a nitric oxide synthase (NOS) inhibitor. Results: Based on our behavioral tests, MPFF (50, 100, 200 mg/kg) effectively reduced some symptoms of VIPN, including mechanical allodynia and hyperalgesia. Notably, TNF-α and IL-6 levels in the DRG tissue of the groups treated with 100 and 200 mg/kg of MPFF showed a significant reduction in inflammatory factors. These results underscore the potential of MPFF and the role of the NO signaling pathway in alleviating neuropathic pain caused by VCR. Conclusions: Micronized purified flavonoid fraction may be an effective treatment for VIPN by reducing mechanical allodynia, thermal hyperalgesia, and TNF-α and IL-6 levels in DRG tissue. The NO pathway may play a role in the effectiveness of MPFF in treating VIPN. | en |
| DOI | https://doi.org/10.5812/ijpr-154455 | en |
| Keyword | Peripheral Neuropathy | en |
| Keyword | Chemotherapy | en |
| Keyword | Hesperidin | en |
| Keyword | Diosmin | en |
| Keyword | Vincristine | en |
| Keyword | Neuroprotective | en |
| Keyword | Anti-inflammatory | en |
| Publisher | Brieflands | en |
| Title | Effect of Micronized Purified Flavonoid Fraction Containing Hesperidin and Diosmin on Vincristine-Induced Neuropathy in Rats; the Role of Nitric Oxide Pathway | en |
| Type | Research Article | en |
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