Curcumin Induces MCF-7 Breast Cancer Cell Apoptosis Through miR-15a Alteration
| Author | Jalal Rezaeidian | en |
| Author | Vahid Naseh | en |
| Author | Maliheh Entezari | en |
| Author | Hakimeh Ziyadi | en |
| Author | Mehrdad Hashemi | en |
| Orcid | Jalal Rezaeidian [0009-0001-1934-7501] | en |
| Orcid | Maliheh Entezari [0000-0003-4191-3351] | en |
| Orcid | Mehrdad Hashemi [0000-0003-0627-6991] | en |
| Issued Date | 2024-12-31 | en |
| Abstract | Background: In recent years, the application of herbal compounds in cancer treatment has shown significant progress. Curcumin (CUR), a natural polyphenol, demonstrates potent anti-cancer effects against various cancers, including breast cancer (BC). Curcumin targets a range of molecular pathways, contributing to the inhibition of cancer cell proliferation, metastasis, and angiogenesis, and promoting apoptosis. Objectives: This study aimed to assess the effect of CUR on BC cells, focusing on alterations in the expression levels of Mir-15a and Bcl-2 through the apoptotic pathway. Methods: The cytotoxicity of CUR was evaluated using an MTT assay. Changes in the expression of Mir-15a and Bcl-2 genes were analyzed by real-time PCR, and cell apoptosis was measured using flow cytometry. Data analysis was conducted using SPSS. Results: The MTT assay results indicated that cell viability decreased as CUR concentration increased (5 – 25 µM). Real-time PCR results showed a significant decrease in the expression of Mir-15a and Bcl-2 (P < 0.05). Flow cytometry findings demonstrated that CUR treatment at the IC50 concentration for 48 hours induced apoptosis in 75.9% of MCF-7 cells. Conclusions: Our study provides a crucial foundation indicating that CUR induces apoptosis in MCF-7 cells by modulating the expression of Mir-15a. | en |
| DOI | https://doi.org/10.5812/ijcm-154265 | en |
| Keyword | Apoptosis | en |
| Keyword | Breast Cancer | en |
| Keyword | Curcumin | en |
| Keyword | Flow Cytometr | en |
| Keyword | MTT Assay | en |
| Keyword | Mir-15a | en |
| Publisher | Brieflands | en |
| Title | Curcumin Induces MCF-7 Breast Cancer Cell Apoptosis Through miR-15a Alteration | en |
| Type | Research Article | en |
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