Detection of IS<i>903</i>, IS<i>26</i> and IS<i>Ecp1</i> Elements in CTX-M-Producing <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i> Isolates From Patients with Leukemia in Iran

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AuthorMahdane Roshanien
AuthorHossein Goudarzien
AuthorAli Hashemien
AuthorAbdollah Ardebilien
AuthorSoroor Erfanimaneshen
AuthorAghil Bahramianen
OrcidAli Hashemi [0000-0002-7258-5541]en
OrcidAbdollah Ardebili [0000-0003-2386-8366]en
Issued Date2018-11-21en
AbstractBackground: The ability of extended spectrum beta-lactamases (ESBLs) production is one of the main mechanisms for the emergence of antibiotic resistance in Escherichia coli and Klebsiella pneumoniae. Objectives: The aim of this study was to evaluate the occurrence of IS903, IS26, and ISEcp1 insertion elements among the CTX-M-producing K. pneumoniae and E. coli isolates from patients with leukemia in Tehran. Methods: Eighty E. coli and K. pneumoniae isolates were recovered from patients admitted to hospitals of Tehran. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and broth microdilution methods. Detection of ESBL producers was evaluated by phenotypic confirmatory test. The presence of IS903, IS26, and ISEcp1 insertion elements in CTX-M-positive E. coli and K. pneumoniae isolates was investigated by PCR-sequencing methods. Results: The rate of resistance of 80 E. coli and K. pneumoniae isolates against the nine antibiotics was as follows: 100% to ampicillin, 15% to amikacin, 51% to ciprofloxacin, 30% to gentamicin, 58% to ceftriaxone, 10% to imipenem, 63% to cefotaxime, 51% to levofloxacin, and 55% to ceftazidime. Using phenotypic confirmatory test revealed that 51 (63.75%) isolates were ESBL producers. The prevalence of CTX-M-1, CTX-M-2, CTX-M-9, CTX-M-8, and CTX-M-25 genes was 87.5%, 13.75%, 23.75%, 10%, and 0%, respectively. IS903, IS26, and ISEcp1 elements were detected in 93.75%, 71.25%, and 100% of the isolates, respectively. Conclusions: This study indicates that the high prevalence of antibiotic resistance, IS and CTX-M-producing E. coli, and K. pneumoniae isolates could be a major concern and highlights the need for infection control measures.en
DOIhttps://doi.org/10.5812/jjm.58929en
Keyword<i>Escherichia coli</i>en
KeywordExtended-Spectrum Beta-Lactamasesen
KeywordInsertion Sequenceen
Keyword<i>Klebsiella pneumoniae</i>en
KeywordLeukemic Patientsen
PublisherBrieflandsen
TitleDetection of IS<i>903</i>, IS<i>26</i> and IS<i>Ecp1</i> Elements in CTX-M-Producing <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i> Isolates From Patients with Leukemia in Iranen
TypeResearch Articleen

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