PCR Assays Based on <i>invA</i> Gene Amplification are not Reliable for <i>Salmonella</i> Detection
| Author | Carolina Resendiz-Nava | en |
| Author | Yajaira Esquivel-Hernandez | en |
| Author | Alejandro Alcaraz-Gonzalez | en |
| Author | Pilar Castaneda-Serrano | en |
| Author | Gerardo M Nava | en |
| Issued Date | 2019-01-31 | en |
| Abstract | Background: Salmonella surveillance relies on invA polymerase chain reaction (PCR) assays for the rapid detection of Salmonella; however, false-positive results have been reported using this method. Objectives: To evaluate the performance and specificity of the published and validated PCR protocols targeting invA gene for the detection of Salmonella. Methods: The performance and specificity of 11 different PCR primer sets were evaluated using Salmonella type strains and Citrobacter spp., Escherichia coli and Serratia spp. isolates recovered during a Salmonella surveillance program. Results: It was revealed that the published PCR protocols using validated primers targeting invA and 16S rRNA genes generated false-positive signals. Importantly, a protocol targeting the ttrA/C genes was able to discriminate Salmonella and non-Salmonella isolates. Conclusions: Detection of Salmonella spp. by means of invA PCR amplification is not reliable. In fact, false-positive results are commonly obtained from Citrobacter, E. coli and Serratia isolates. It is recommended to use other loci, such as ttrA/C genes, for the accurate and reliable detection of Salmonella. | en |
| DOI | https://doi.org/10.5812/jjm.68764 | en |
| Keyword | <i>Salmonella</i> | en |
| Keyword | <i>invA</i> | en |
| Keyword | PCR | en |
| Keyword | Detection | en |
| Keyword | <i>Citrobacter</i> | en |
| Keyword | <i>16S rRNA</i> | en |
| Keyword | <i>ttrA</i> | en |
| Keyword | <i>ttrC</i> | en |
| Publisher | Brieflands | en |
| Title | PCR Assays Based on <i>invA</i> Gene Amplification are not Reliable for <i>Salmonella</i> Detection | en |
| Type | Brief Report | en |