MiR-135b Induces Osteosarcoma Invasion by the Modulation of FOXO-1 and c-Myc
| Author | Behnoosh Tahbazlahafi | en |
| Author | Malihe Paknejad | en |
| Author | Shiva Shahmohamadnejad | en |
| Author | Alireza Mirzaei | en |
| Author | Khodamorad Jamshidi | en |
| Author | Ehsan Khalili | en |
| Orcid | Behnoosh Tahbazlahafi [0000-0002-3320-6828] | en |
| Orcid | Malihe Paknejad [0000-0001-8050-2534] | en |
| Orcid | Shiva Shahmohamadnejad [0000-0001-5303-7374] | en |
| Orcid | Alireza Mirzaei [0000-0002-1569-6130] | en |
| Orcid | Khodamorad Jamshidi [0000-0001-9475-5866] | en |
| Orcid | Ehsan Khalili [0000-0001-9968-4374] | en |
| Issued Date | 2020-08-11 | en |
| Abstract | Background: Osteosarcoma (OS) is the most common type of bone malignancy. Many studies have attempted to find the association between microRNAs and cancer-associated processes. Alterations in miRNA expression through genetic or epigenetic changes, impairment of transcription factors, and ectopic expression of miRNAs induce the development and progression of cancer. Although miR-135b has been thoroughly documented as an oncogene in the majority of studies, some controversies remain about the conflicting role of miR-135b as a tumor-suppressor. Objectives: The present study aimed at investigating the oncogenic and/or tumor-suppressing role of miR-135b in human OS. Methods: In this study, 21 OS tissue samples, along with 21 adjacent bone tissues (normal) as control specimens were collected to analyze the expression of miR-135b. The Saos2 cell-line was transiently transfected with the miR-135b mimic and inhibitor to assess its effect on two critical transcription factors, namely FOXO-1 and c-Myc. qRT-PCR was performed to quantify the expression of miR-135b in both OS tissues and the Saos2 cell-line. The MTT, cell migration, and cell invasion assays were used to characterize the miR-135b function. The western blot analysis was carried out to monitor the targets of miR-135b. Finally, the changes in cellular functions such as migration and invasion, following the transfection of miR-135b mimic and inhibitor, were verified. Results: The results showed that in comparison with the adjacent normal bone tissues, the expression of miR-135b was higher in OS tissue samples, which inversely correlated with the expression rate of FOXO-1, whereas the expression of c-Myc had a direct relationship to miR-135b expression. Functionally, the miR-135b mimic led to an increase in cell proliferation, invasion, and migration of OS cancer cells. Conclusions: MiR-135b induces the proliferation and invasion of OS cells by the degradation of FOXO-1 and upregulation of c-Myc. | en |
| DOI | https://doi.org/10.5812/ijcm.101577 | en |
| Keyword | Osteosarcoma | en |
| Keyword | FOXO-1 | en |
| Keyword | c-Myc | en |
| Keyword | miR-135b | en |
| Keyword | Cell Invasion | en |
| Publisher | Brieflands | en |
| Title | MiR-135b Induces Osteosarcoma Invasion by the Modulation of FOXO-1 and c-Myc | en |
| Type | Research Article | en |