Immunocytochemical Study of p53 Protein in Exfoliated Cells of Oral Mucosa in Patients With Type 2 Diabetes
| Author | Zahra Heidari | en |
| Author | Hamidreza Mahmoudzade Sagheb | en |
| Author | Azam Asemi Rad | en |
| Author | Mahmoud Ali Keikhaee | en |
| Issued Date | 2015-01-01 | en |
| Abstract | Background: There is a potential risk for malignancy in patients with diabetes mellitus. Theoretically, a noninvasive method for detection of precancerous changes can be conducted on oral exfoliated cell smears. Objectives: In this study, the immunocytochemistry of p53 in exfoliated oral mucosal cells in patients with type 2 diabetes was investigated. Patients and Methods: Oral mucosal smears were prepared from 50 patients with type 2 diabetes (D) and 50 healthy participants (C). The immunocytochemistry of p53 was assessed according to the kit instructions on oral smears. The Mann-Whitney nonparametric test was used to compare the p53 expression in D and C groups. Results: The results showed a significant increase in p53 expression in exfoliated cells of oral mucosa in D group compared with C group (P < 0.05). There was a significant correlation between the intensity of p53 expression and level of fasting blood sugar (FBS) (r = 0.816, P < 0.001), as well as between the percentage of p53 positive cells and FBS. The nucleus area (NA) and the cytoplasm to nuclear ratio (CNR) had significant correlations with p53 expression (r = 0.272, P = 0.08) NA, and CNR (r = -0.434, P = 0.005). Conclusions: This study showed that there was a significant expression of p53 in oral mucosal exfoliated cells of group D patients compared with C group. It seems that this noninvasive technique could be useful to detect premalignancy changes of oral mucosa as well as for early detection of malignancies. | en |
| DOI | https://doi.org/10.17795/gct-24881 | en |
| Keyword | Diabetes Mellitus | en |
| Keyword | Immunocytochemistry | en |
| Keyword | Buccal Mucosa | en |
| Keyword | p53 Genes | en |
| Publisher | Brieflands | en |
| Title | Immunocytochemical Study of p53 Protein in Exfoliated Cells of Oral Mucosa in Patients With Type 2 Diabetes | en |
| Type | Research Article | en |
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