Immunovirological Assessment of HIV-Infected Patients and Phylogenetic Analysis of the Virus in Northeast of Iran
| Author | Shadi Abbasnia | en |
| Author | Tahereh Hassannia | en |
| Author | Nazila Ariaee | en |
| Author | Faezeh Sabet | en |
| Author | Kobra Razavi Pashabayg | en |
| Author | Hossain Keyvani | en |
| Author | M, Reza Sarv-Ghad | en |
| Author | Aghigh Ziaeemehr | en |
| Author | Faezeh Fakor | en |
| Author | Narges Valizadeh | en |
| Orcid | Faezeh Sabet [0000-0001-5422-1077] | en |
| Issued Date | 2021-03-31 | en |
| Abstract | Background: The reliable laboratory tests, co-infection with other tumor viruses, and determining the genetic types are very important for therapy and monitoring of the clinical status of human immunodeficiency virus (HIV)-infected subjects. Objectives: This study evaluated the co-infection of HIV with hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-cell leukemia virus type 1 (HTLV-1), the viral load, the progression of infection, and its correlation with the clinical status. Methods: Twenty HIV-infected cases were assessed for T cell subpopulations, HBV, HCV, and HTLV-1 co-infection, as well as the viral load. For phylogenetic relationships analysis, the HIV-c2-v5 fragment and p17 of gag were amplified, sequenced, and then clustered using phylogenetic analysis by MEGA software with maximum-likelihood. Results: The quantity of HIV viral load by qRT-PCR (TaqMan) and Cobas-Amplicor monitor test had a very strong correlation (R = 0.881, P < 0.0001). A significant negative correlation was also found between CD4+ cell count and Cobas-Amplicor (R = -0.41, P = 0.06). A significant negative correlation was also found between CD4+ cell count and Cobas-Amplicor (R = -0.41, P = 0.06) with HIV monitor test results (R = -0.41, P = 0.06). The phylogenetic analysis for p17 regions in gag and c2-v5 in env genes showed that all subjects had AD genotype. The co-infection of the HIV subjects with HBV, HCV, and HTLV-1 was 75%, 75%, and 15%, respectively. A direct correlation was observed between CD8+ and HIV-HTLV-1 co-infection. Conclusions: The results showed that HIV CRF35-AD, (M group) is more frequent in the northeast of Iran, and both real-time quantification methods were reliable for monitoring the HIV-1 viral load. In addition, the transmission rate of HTLV-1 is lower than HBV and HCV among drug abusers. | en |
| DOI | https://doi.org/10.5812/jjm.112123 | en |
| URI | https://brieflands.com/journals/jjm/articles/112123 | en |
| Keyword | HIV | en |
| Keyword | Phylogenies | en |
| Keyword | Subtype | en |
| Keyword | Coinfection | en |
| Keyword | Iran | en |
| Publisher | Brieflands | en |
| Title | Immunovirological Assessment of HIV-Infected Patients and Phylogenetic Analysis of the Virus in Northeast of Iran | en |
| Type | Research Article | en |