Comparative Prevalence of <i>bla</i><sub>CTX-M-15 </sub>Gene with Virulence Genes and Serotypes in <i>Klebsiella pneumoniae</i>
| Author | Hesam Alizade | en |
| Author | Maziar Jajarmi | en |
| Author | Mohammad Reza Aflatoonian | en |
| Author | Davood Kalantar-Neyestanaki | en |
| Author | Saeed Shoja | en |
| Author | Reza Ghanbarpour | en |
| Orcid | Saeed Shoja [0000-0001-8019-5634] | en |
| Orcid | Reza Ghanbarpour [0000-0002-8568-5146] | en |
| Issued Date | 2018-04-30 | en |
| Abstract | Background: A newly emerged hypervirulent strain of Klebsiella pneumoniae has caused great concern globally; however, its molecular characteristics have been rarely reported in Iran. Objectives: The goal of this study was to detect the virulence determinants and serotypes of K. pneumoniae and to evaluate the association among selected virulence traits and blaCTX-M-15 gene in southeastern Iran. Methods: One hundred and three non-duplicate K. pneumoniae strains were isolated from clinical samples. The isolates were identified by standard bacteriological tests. Confirmed isolates were examined to detect a selection of virulence genes (wabG, rmpA and iucB) and serotypes (K1, K2, K5 and K20) by PCR. The isolates were studied foe the presence of beta-lactamase (blaCTX-M-15) gene. SPSS software (version 19.0) was used for data analysis. Results: Among the 103 K. pneumoniae isolates, 61 (59.2%) isolates were positive for wabG, 4 (3.9%) for iucB and 3 (2.9%) for rmpA genes. The presence of K20 in 3.9% (4/103) of the isolates represented the most prevalent. Only 3 (2.9%) isolates possessed the K1 serotype, while K2 and K5 serotypes were not detected in any isolate. The blaCTX-M-15 gene was detected in 47 (45.6%) isolates. blaCTX-M-15-positive isolates showed a higher prevalence of wabG among the studied isolates (P < 0.05). Conclusions: Our data indicate a correlation between presence of virulence gene and blaCTX-M-15 in K. pneumonia isolates. Further research should be undertaken to unravel aspects of both virulence factors and antibiotic resistance which may probably contribute to managing future spread of infectious diseases. | en |
| DOI | https://doi.org/10.5812/jjm.61285 | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | Serogroup | en |
| Keyword | Virulence Factors | en |
| Keyword | Iran | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Publisher | Brieflands | en |
| Title | Comparative Prevalence of <i>bla</i><sub>CTX-M-15 </sub>Gene with Virulence Genes and Serotypes in <i>Klebsiella pneumoniae</i> | en |
| Type | Research Article | en |