Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
| Author | Seyed Hossein Abdollahi |
| Author | Fateme Ayoobi |
| Author | Hossein Khorramdelazad |
| Author | Behzad Nasiri Ahmadabadi |
| Author | Mohammadtaghi Rezayati |
| Author | Mohammad Kazemi Arababadi |
| Author | Mohammad Zare-Bidaki |
| Issued Date | 2015-05-31 |
| Abstract | Background:: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. Objectives:: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β). Materials and Methods:: Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed. Results:: Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA. Conclusions:: Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization. |
| DOI | https://doi.org/10.5812/jjm.8(5)2015.17802 |
| Keyword | Toxoplasma |
| Keyword | Immunization |
| Keyword | Transforming Growth Factor-beta |
| Keyword | Excretory-Secretory Antigen |
| Publisher | Brieflands |
| Title | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
| Type | Research Article |
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