Royal Jelly Consumption Controls Endurance Training-Induced Increases in ANP and BNP in Brain Tissue of Rats with Experimental Autoimmune Encephalomyelitis.

Abstract

Background and Objectives: Atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) changes are known to be associated with the occurrence of cardiac diseases in individuals with muscular dystrophy (MS). Therefore, the aim of this study was to investigate the effect of endurance training (ET) and royal jelly (RJ) consumption on ANP and BNP in an experimental autoimmune encephalomyelitis (EAE) model. Methods: In this experimental study, 49 female rats with EAE (induced by complete Freund’s adjuvant) were divided into seven groups: EAE, Sham (Sh), 50 mg/kg RJ (RJ50), RJ100, ET, ET+RJ50, and ET+RJ100. Additionally, seven healthy control rats (HC) were included to evaluate the effect of disease induction on the research variables. ET was performed for five weeks, four sessions per week at a speed of 11 - 15 m/min for 30 minutes per session. RJ was injected intraperitoneally daily at doses of 50 and 100 mg/kg, dissolved in normal saline. All outcome measurements of ANP and BNP levels in brain tissue were performed immediately after the 5-week intervention period using validated ELISA kits according to the manufacturers’ instructions. Statistical analysis included the Shapiro-Wilk test for normality, one-way ANOVA for group comparisons, and Tukey’s post-hoc test for pairwise comparisons. Results: In the ET group, ANP levels were lower than in the EAE group. In the RJ100 and ET+RJ100 groups, ANP and BNP levels were lower than in the EAE group. Also, in the RJ50 group, ANP levels were lower than in the EAE group. ANP levels in the ET+RJ100 group were lower than in the RJ50 and RJ100 groups; Also, in the ET+RJ100 group were lower than ET+RJ50 group as well as BNP levels in the ET+RJ50 group were lower than EAE group (P ≤ 0.05). Conclusion: Although ET leads to an increase in BNP and ANP, and RJ with different doses leads to a decrease in them, the use of RJ along with ET, in addition to the efficiency of the effect of training on cardiac and cerebral natriuretics, controls their excessive increase.