Effects of Phenylglyoxal and N-ethylmaleimide Concentration on Mycophenolic Acid Production by <i>Penicillium brevi-compactum </i>ATCC16024
Author | Fatemeh Ardestani | en |
Issued Date | 2016-10-31 | en |
Abstract | Mycophenolic acid is a secondary extracellular metabolite of Penicillium strains with numerous pharmaceutical properties such as antibiotic and immunosuppressive uses. The aim of this work is the survey of the effect of phenylglyoxal and n-ethylmaleimide concentration in culture medium on mycophenolic acid production by Penicillium brevi-compactum ATCC16024 was investigated. Batch submerged fermentation was performed in 250 mL shake flasks at 24 °C and 200 rpm in a rotary shaker for 300 h using a basic culture medium containing different concentrations of phenylglyoxal and n-ethylmaleimide ranged from 0 to 20 mg. L-1. For the basic medium without any amounts of phenylglyoxal and n-ethylmaleimide (control), maximum MPA production, product yield and productivity of process was in order, 1.5042 g. L-1, 20.3 mg. g-1 consumed glucose and 5.37 mg. L-1h-1. Maximum produced MPA of 2.9032 g. L-1, MPA yield of 39.23 mg. g-1 of consumed glucose, productivity of 10.37 mg. L-1 h-1 and total enhancement of 93.11% was obtained when the culture medium was contained 18 mg. L-1 of phenylglyoxal, represented more than 93% raising in compare to control. Maximum MPA concentration, yield and productivity in order was obtained 3.1123 g. L-1, 42.06 mg. g-1 of consumed glucose and 11.11 mg. L-1 h-1, with using 6 mg. L-1 of n-ethylmaleimide. N-ethylmaleimide was caused to 2.138 folds (106.89%) increase in MPA production by P. brevi-compactum ATCC16024. | en |
DOI | https://doi.org/10.22037/ijpr.2016.1919 | en |
Keyword | Antibiotic | en |
Keyword | Aerobic fermentation | en |
Keyword | Immunosuppressive | en |
Keyword | Mycophenolic acid | en |
Keyword | <i>Penicillium brevi-compactum</i> | en |
Publisher | Brieflands | en |
Title | Effects of Phenylglyoxal and N-ethylmaleimide Concentration on Mycophenolic Acid Production by <i>Penicillium brevi-compactum </i>ATCC16024 | en |
Type | Original Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- ijpr-15-925.pdf
- Size:
- 426.27 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF