Screening and Isolation of Natural Antioxidants from <i>Acer Ginnala</i> Max by TLC-MS/MS Guided Bioautographic Method
| Author | Jia-Ming Sun | en |
| Author | Yinan Guo | en |
| Author | Jie Zhang | en |
| Author | Hui Zhang | en |
| Author | Zhe Lin | en |
| Issued Date | 2019-04-30 | en |
| Abstract | A rapid and simple method has been developed for the screening and identification of natural antioxidants from the leaves of Acerginnala Maxim (AG). The process is that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the white yellow spots of compounds with potential antioxidant effects will be significantly observed on the thin-layer chromatography (TLC), and possible structures will be presumed by the ESI/MS technique. Using the improved approach, 6 compounds in the AG extract were found to possess a potential antioxidant activity. They were speculated as quercetin-3-O-α-L-(3"-galloyl)-rhamnoside (1), quercetin-3-O-α-L-(2"-galloyl)-rhamnoside (2), quercetin-3-O-α-L-(2"-galloyl)-arabinopyranoside (3), acertannin (4), gallic acid (5), and methyl gallate (6). In addition, we were still found that compounds 2, 3, and 5 had favorable antioxidant activity from the scannogram of the DPPH reaction plate. As a result, the isolated 6 compounds structures were in accordance with the presumed structures. Furthermore, the free radical scavenging capacities of the available identified compounds were also investigated. Compounds 2, 3, and 5 showed significant DPPH.Scavenging capacities, with IC50 values of 2.83 μg/mL, 2.34 μg/mL, and 1.86μg/mL, respectively. The results indicated that this newly improved method could be widely applied for rapid screening and identification of natural antioxidants from Chinese herbal medicines. | en |
| DOI | https://doi.org/10.22037/ijpr.2019.1100690 | en |
| Keyword | Antioxidants | en |
| Keyword | Acer ginnala Max. | en |
| Keyword | TLC | en |
| Keyword | DPPH | en |
| Keyword | ESI/MS | en |
| Publisher | Brieflands | en |
| Title | Screening and Isolation of Natural Antioxidants from <i>Acer Ginnala</i> Max by TLC-MS/MS Guided Bioautographic Method | en |
| Type | Original Article | en |
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