Human Papillomavirus (HPV16 and HPV18) Infection; Pathogenesis, Vaccination, and Detection by Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick Tests: A Review
| Author | Farzane Kargar | en |
| Author | Mohammad Rahmati | en |
| Author | Marzieh Jamalidoust | en |
| Author | Mojtaba Mortazavi | en |
| Author | Amir Savardashtaki | en |
| Author | Morteza Milani | en |
| Author | Amir Vahedi | en |
| Orcid | Mohammad Rahmati [0000-0001-7791-3287] | en |
| Orcid | Marzieh Jamalidoust [0000-0002-7034-1236] | en |
| Orcid | Mojtaba Mortazavi [0000-0003-2376-3898] | en |
| Orcid | Amir Savardashtaki [0000-0003-1700-9426] | en |
| Orcid | Morteza Milani [0000-0002-9822-703X] | en |
| Orcid | Amir Vahedi [0000-0001-5586-3485] | en |
| Issued Date | 2023-04-30 | en |
| Abstract | Context: Human papillomavirus (HPV) infections contribute to the cause of 15% - 20% of all human cancers. This review aims to examine and provide updated information on various aspects, with a particular focus on topics that are of interest to dermatologists. Evidence Acquisition: Using published studies, the pathogenicity of HPV was investigated. Subsequently, the existing vaccines were explored, followed by a review of the loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) method. Results: For HPV detection, the polymerase chain reaction (PCR), self-sustained sequence replication (3SR), strand displacement amplification (SDA), and nucleic acid sequence-based amplification (NASBA) were used. These methods can have good detection but still have problems. In comparison with nested PCR, the detection of HPV16 and HPV18 using LAMP-LFD has higher sensitivity. Conclusions: Loop-mediated isothermal amplification-lateral flow dipstick is a simple and rapid method for the specific and sensitive detection of HPV. Thus, along with the previous HPV16 and HPV18 diagnostic tools, LAMP-LFD might be useful in field studies or local hospitals. | en |
| DOI | https://doi.org/10.5812/apid-140119 | en |
| Keyword | HPV16 | en |
| Keyword | HPV18 | en |
| Keyword | Infection | en |
| Keyword | Pathogenesis | en |
| Keyword | Vaccination | en |
| Keyword | LAMP | en |
| Keyword | LFD | en |
| Publisher | Brieflands | en |
| Title | Human Papillomavirus (HPV16 and HPV18) Infection; Pathogenesis, Vaccination, and Detection by Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick Tests: A Review | en |
| Type | Review Article | en |