Immunomodulatory Effects of Bee Venom in Human Synovial Fibroblast Cell Line
| Author | Ebrahim Mohammadi | en |
| Author | Hossein Vatanpour | en |
| Author | Farshad H Shirazi | en |
| Issued Date | 2015-01-31 | en |
| Abstract | As in Iranian traditional medicine, bee venom (BV) is a promising treatment for the rheumatoid arthritis (RA) which is considered as a problematic human chronic inflammatory disease in the present time. Smoking is considered to be a major risk factor in RA onset and severity. The main aim of this study is to investigate the effects of BV on cigarette smoke-induced inflammatory response in fibroblast-like synoviocytes (FLS). Cytotoxicity of cigarette smoke condensate (CSC) and bee venom were determined by the tetrazolium (MTT) method in cultured synovial fibroblastes. The expression of interleukin-1β and sirtuin1 mRNA were analyzed by SYBR green real-time quantitative PCR. Differences between the mean values of treated and untreated groups were assessed by student t-test. Based on MTT assay, CSC and BV did not exert any significant cytotoxic effects up to 40 µg/mL and 10 µg/mL, respectively. Our results showed that interleukin-1β mRNA level was significantly up-regulated by CSC treatments in LPS-stimulated synoviocytes in a dose-dependent manner. Conversely, the expressions of IL-1β and Sirt1 were up-regulated even in lower concentrations of BV and attenuated at higher concentrations. Also, BV attenuated the CSC-induced and LPS-induced inflammatory responses in synovial fibroblasts. Our results support the epidemiological studies indicating pro-inflammatory effects of CSC and anti-inflammatory effects of BV on FLS cell line. | en |
| DOI | https://doi.org/10.22037/ijpr.2015.1613 | en |
| Keyword | Bee venom | en |
| Keyword | Cigarette | en |
| Keyword | Cytokine | en |
| Keyword | Sirt1 | en |
| Keyword | Fibroblast-like synoviocytes | en |
| Publisher | Brieflands | en |
| Title | Immunomodulatory Effects of Bee Venom in Human Synovial Fibroblast Cell Line | en |
| Type | Original Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- ijpr-14-313.pdf
- Size:
- 644.68 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF